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Scale-up of product...
Scale-up of production of bacterial nanocellulose using submerged cultivation
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- Chen, Genqiang (författare)
- Umeå universitet,Kemiska institutionen,Department of Bioengineering, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, , Shanghai, China,Shanghai University, China; Umeå University, Sweden
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- Wu, Guochao (författare)
- Umeå universitet,Kemiska institutionen,Umeå University, Sweden
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- Alriksson, Björn (författare)
- RISE,Processum
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- Chen, Lin (författare)
- Donghua University, China
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- Wang, Wei (författare)
- Donghua University, China
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- Jönsson, Leif J. (författare)
- Umeå universitet,Kemiska institutionen,Umeå University, Sweden
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- Hong, Feng (författare)
- Umeå University, Sweden
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(creator_code:org_t)
- 2018-06-13
- 2018
- Engelska.
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Ingår i: Journal of chemical technology and biotechnology (1986). - : Wiley. - 0268-2575 .- 1097-4660. ; 93:12, s. 3418-3427
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- BACKGROUND: More extensive utilization of bacterial nanocellulose (BNC) is severely restricted by the low efficiency and small scale of the traditional static cultivation. Submerged fermentation in stirred-tank reactors (STRs) is potentially favourable for large-scale production of BNC, but scale-up of cultivation remains challenging. Even though the STR is most commonly used for submerged cultivation in the fermentation industry, there are few previous attempts to scale-up production of BNC to pilot scale using an STR. Furthermore, the question of how scale-up of submerged cultivation affects the properties of the BNC has received very little attention. RESULTS: Four strains were compared in 250-mL shake flasks. Strain DHU-ATCC-1 displayed the highest volumetric productivity, 0.56 g L−1 d−1, and was then cultivated in a 400-mL STR, showing a similar productivity of 0.55 g L−1 d−1. Scale-up using a 75-L STR pilot bioreactor resulted in enhancement of the BNC production rate from 0.056 g d−1 in the shake flasks to 17.3 g d−1 in the 75-L STR, although the productivity decreased to 0.43 g L−1 d−1. During scale-up from shake flasks to 400-mL STR and further on to 75-L STR, the BNC fibers formed more bundles, whereas the fiber diameter decreased from 25.6 to 21.7 nm. The BNC from the 75-L STR exhibited a higher degree of polymerization, specifically 3230, higher degree of crystallinity, specifically 83%, larger crystallites, and improved strength including higher tensile energy absorption index and superior stretch at break. CONCLUSION: It is possible to enhance BNC production, and maintain or improve its properties when scaling up submerged cultivation in STRs.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- bacterial nanocellulose
- Komagataeibacter xylinus
- mechanical property
- productivity
- stirred-tank reactor
- structure
- Bottles
- Cellulose
- Chemical industry
- Fermentation
- Mechanical properties
- Structure (composition)
- Tanks (containers)
- Degree of crystallinity
- Degree of polymerization
- Large scale productions
- Stirred tank reactors
- Submerged fermentation
- Tensile energy absorption index
- Volumetric productivity
- Nanocellulose
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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