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The influence of as...
The influence of aspirin on release of eoxin C4, leukotriene C4 and 15-HETE, in eosinophilic granulocytes isolated from patients with asthma
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- James, Anna (författare)
- Karolinska Institutet
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Daham, Kameran (författare)
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Backman, Linda (författare)
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Brunnström, Åsa (författare)
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Tingvall, Tove (författare)
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- Kumlin, Maria (författare)
- Sophiahemmet Högskola
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Edenius, Charlotte (författare)
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- Dahlén, Sven-Erik (författare)
- Karolinska Institutet
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- Dahlén, Barbro (författare)
- Karolinska Institutet
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- Claesson, Hans-Erik (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2013-07-31
- 2013
- Engelska.
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Ingår i: International Archives of Allergy and Immunology. - : S. Karger AG. - 1018-2438 .- 1423-0097. ; 162:2, s. 135-42
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: The effect of aspirin on the release of key arachidonic acid metabolites in activated eosinophils from subjects with aspirin-intolerant asthma (AIA) has not been investigated previously, despite the characteristic eosinophilia in AIA. Methods: Peripheral blood eosinophils were isolated from four groups of subjects: healthy volunteers (HV; n = 8), mild asthma (MA; n = 8), severe asthma (SA; n = 9) and AIA (n = 7). In the absence or presence of lysine-aspirin, eosinophils were stimulated with arachidonic acid or calcium ionophore to trigger the 15-lipoxygenase-1 (15-LO) and 5-lipoxygenase (5-LO) pathways, respectively. 15(S)-hydroxy-eicosatetraenoic acid (15-HETE) and eoxin C4 (EXC4) were measured as 15-LO products and leukotriene (LT)C4 as a product of the 5-LO pathway. Results: Activated eosinophils from patients with SA and AIA produced approximately five times more 15-HETE than eosinophils from HV or MA patients. In the presence of lysine-aspirin, eosinophils from AIA, MA and SA patients generated higher levels of 15-HETE than in the absence of lysine-aspirin. Furthermore, in the presence of lysine-aspirin, formation of EXC4 was also significantly increased in eosinophils from AIA patients, and LTC4 synthesis was increased both in AIA and SA patients. Conclusions: Taken together, this study shows an increased release of the recently discovered lipid mediator EXC4, as well as the main indicator of 15-LO activity, 15-HETE, in activated eosinophils from severe and aspirin-intolerant asthmatics, and also elevated EXC4 and LTC4 formation in eosinophils from AIA patients after cellular activation in the presence of lysine-aspirin. The findings support a pathophysiological role of the 15-LO pathway in SA and AIA.
Nyckelord
- Asthma
- Arachidonic acid
- Lipoxygenase
- Eoxin
- Leukotriene
- Aspirin hypersensitivity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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James, Anna
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Daham, Kameran
-
Backman, Linda
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Brunnström, Åsa
-
Tingvall, Tove
-
Kumlin, Maria
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visa fler...
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Edenius, Charlot ...
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Dahlén, Sven-Eri ...
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Dahlén, Barbro
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Claesson, Hans-E ...
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visa färre...
- Artiklar i publikationen
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International Ar ...
- Av lärosätet
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Sophiahemmet Högskola
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Karolinska Institutet