Sökning: id:"swepub:oai:DiVA.org:su-104123" >
Quantification and ...
Quantification and repair of psoralen-induced interstrand crosslinks in human cells
-
- Vare, Daniel (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Johansson, Fredrik (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Persson, Jan-Olov (författare)
- Stockholms universitet,Matematiska institutionen
-
visa fler...
-
- Erixon, Klaus (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Jenssen, Dag (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- Engelska.
-
Ingår i: Toxicology Letters. - : Elsevier BV. - 0378-4274 .- 1879-3169. ; 226:3, s. 343-350
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Bi-functional alkylating agents that cause crosslinks are commonly used in chemotherapy. However, there is no conclusive knowledge for human cells regarding the number of induced interstrand crosslinks (ICLs) and the unhooking rate when the lesion is removed from one of the DNA strand. Using a newly developed method, we quantified the number of induced ICLs for the five furocoumarins; psoralen, 5-methoxypsoralen, 8-methoxypsoralen, tri-methoxypsoralen and angelicin. In quantitative terms, the results were in agreement with the values found by others. In kinetic studies using mammalian cells, we found that half of the psoralen-induced ICLs were unhooked within 2.5 h. The rate in normal human diploid fibroblasts was found to be 20,000 ICLs/h/cell. In comparison to survival, 2500 ICLs per cell led to 50% toxicity, indicating that the unhooking of the ICLs is not the crucial step for ICL tolerance. Surprisingly, only 3500 ICLs per cell corresponded to a significant delay in the replication fork elongation. The results indicate involvements of additional pathway(s) for the delay since the effect on replication elongation could be monitored when only 10% of the replication forks encounter an ICL.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- DNA repair
- DNA interstrand crosslinks
- ICL
- Psoralen
- Furocoumarins
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas