Chemical analysis of complex mixtures with regard to potentially genotoxic organic compounds

• This thesis describes different methods for the extraction, fractionation, and analysis of particle adsorbed organic compounds. The utility of capillary gas chromatography mass spectrometry (GC-MS) for the analysis of polycyclic aromatic compounds (PAC) is demonstrated. Negative ion chemical ionization mass spectrometry (NCI-MS) was used for the selective detection of organic bromides in automobile exhaust extracts. Papers I and II are concerned with the characterization of different sources with respect to their emissions of polynuclear aromatic hydrocarbons (PAH). In Paper III vacuum sublimation is compared to Soxhlet extraction as a means for the extraction of PAC from carbonaceous material.Papers IV, VI, and VII describe a combination of chemical analysis and biological testing. Complex mixtures of PAC obtained from carbon black and from gasoline vehicle exhaust particulates were fractionated into compound classes and tested for genotoxic activity in the Salmonella/microsome test. Fractionation was performed with low pressure liquid chromatography on silica gel, and high-performance liquid chromatography (HPLC) using both normal and reversed phase columns. The genotoxic effects of the different fractions are discussed in relation to the results from the chemical analysis.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

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