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A genome-wide IR-in...
A genome-wide IR-induced RAD51 foci RNAi screen identifies CDC73 involved in chromatin remodeling for DNA repair
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- Herr, Patrick (författare)
- Karolinska Institutet
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Lundin, Cecilia (författare)
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Evers, Bastiaan (författare)
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Ebner, Daniel (författare)
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Bauerschmidt, Christina (författare)
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Kingham, Guy (författare)
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Palmai-Pallag, Timea (författare)
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- Mortusewicz, Oliver (författare)
- Karolinska Institutet
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- Frings, Oliver (författare)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för biokemi och biofysik
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- Sonnhammer, Erik (författare)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för biokemi och biofysik
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- Helleday, Thomas (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2015-12-01
- 2015
- Engelska.
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Ingår i: Cell discovery. - : Springer Science and Business Media LLC. - 2056-5968. ; 1
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- To identify new regulators of homologous recombination repair, we carried out a genome-wide short-interfering RNA screen combined with ionizing irradiation using RAD51 foci formation as readout. All candidates were confirmed by independent short-interfering RNAs and validated in secondary assays like recombination repair activity and RPA foci formation. Network analysis of the top modifiers identified gene clusters involved in recombination repair as well as components of the ribosome, the proteasome and the spliceosome, which are known to be required for effective DNA repair. We identified and characterized the RNA polymerase II-associated protein CDC73/Parafibromin as a new player in recombination repair and show that it is critical for genomic stability. CDC73 interacts with components of the SCF/Cullin and INO80/NuA4 chromatin-remodeling complexes to promote Histone ubiquitination. Our findings indicate that CDC73 is involved in local chromatin decondensation at sites of DNA damage to promote DNA repair. This function of CDC73 is related to but independent of its role in transcriptional elongation.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- genome-wide siRNA screen
- RAD51
- homologous recombination
- tumor suppressor
- CDC73
- Parafibromin
- DNA repair
- chromatin remodeling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Herr, Patrick
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Lundin, Cecilia
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Evers, Bastiaan
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Ebner, Daniel
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Bauerschmidt, Ch ...
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Kingham, Guy
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visa fler...
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Palmai-Pallag, T ...
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Mortusewicz, Oli ...
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Frings, Oliver
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Sonnhammer, Erik
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Helleday, Thomas
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Artiklar i publikationen
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Cell discovery
- Av lärosätet
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Stockholms universitet
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Karolinska Institutet