Semiquinone-induced Maturation of Bacillus anthracis Ribonucleotide Reductase by a Superoxide Intermediate

• Background: Activation of ribonucleotide reductase Ib depends on the flavodoxin-like maturase NrdI.Results: The redox properties of Bacillus anthracis NrdI allow isolation of the semiquinone state, NrdI(sq), which can catalyze formation of the manganese-tyrosyl radical cofactor.Conclusion: The maturation capacity of NrdI(sq) provides evidence that Mn-NrdF is activated via a superoxide radical.Significance: Novel antibiotics may be designed to selectively target the maturation mechanism.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Bacillus

Free Radicals

Manganese

Ribonucleotide Reductase

Superoxide Ion

NrdF

NrdI

Cyclic Voltammetry

Maturase

Semiquinone

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