Sökning: id:"swepub:oai:DiVA.org:su-180484" >
Spatiotemporal mapp...
Spatiotemporal mapping of RNA editing in the developing mouse brain using in situ sequencing reveals regional and cell-type-specific regulation
-
- Lundin, Elin (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
-
- Wu, Chenglin (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
-
- Widmark, Albin (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
visa fler...
-
Behm, Mikaela (författare)
-
- Hjerling-Leffler, Jens (författare)
- Karolinska Institutet
-
- Daniel, Chammiran (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Öhman, Marie (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Nilsson, Mats (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
-
visa färre...
-
(creator_code:org_t)
- 2020-01-14
- 2020
- Engelska.
-
Ingår i: BMC Biology. - : Springer Science and Business Media LLC. - 1741-7007. ; 18:1
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background Adenosine-to-inosine (A-to-I) RNA editing is a process that contributes to the diversification of proteins that has been shown to be essential for neurotransmission and other neuronal functions. However, the spatiotemporal and diversification properties of RNA editing in the brain are largely unknown. Here, we applied in situ sequencing to distinguish between edited and unedited transcripts in distinct regions of the mouse brain at four developmental stages, and investigate the diversity of the RNA landscape. Results We analyzed RNA editing at codon-altering sites using in situ sequencing at single-cell resolution, in combination with the detection of individual ADAR enzymes and specific cell type marker transcripts. This approach revealed cell-type-specific regulation of RNA editing of a set of transcripts, and developmental and regional variation in editing levels for many of the targeted sites. We found increasing editing diversity throughout development, which arises through regional- and cell type-specific regulation of ADAR enzymes and target transcripts. Conclusions Our single-cell in situ sequencing method has proved useful to study the complex landscape of RNA editing and our results indicate that this complexity arises due to distinct mechanisms of regulating individual RNA editing sites, acting both regionally and in specific cell types.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Single-cell resolution
- RNA editing
- Spatially resolved transcriptomics
- Brain development
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas