Sökning: id:"swepub:oai:DiVA.org:su-197330" >
Global distribution...
Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods
-
Pierella Karlusich, Juan José (författare)
-
Pelletier, Eric (författare)
-
Lombard, Fabien (författare)
-
visa fler...
-
Carsique, Madeline (författare)
-
Dvorak, Etienne (författare)
-
Colin, Sébastien (författare)
-
Picheral, Marc (författare)
-
Cornejo-Castillo, Francisco M. (författare)
-
Acinas, Silvia G. (författare)
-
Pepperkok, Rainer (författare)
-
Karsenti, Eric (författare)
-
de Vargas, Colomban (författare)
-
Wincker, Patrick (författare)
-
Bowler, Chris (författare)
-
- Foster, Rachel A. (författare)
- Stockholms universitet,Institutionen för ekologi, miljö och botanik
-
visa färre...
-
(creator_code:org_t)
- 2021-07-06
- 2021
- Engelska.
-
Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 12:1
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://www.nature.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Nitrogen fixation has a critical role in marine primary production, yet our understanding of marine nitrogen-fixers (diazotrophs) is hindered by limited observations. Here, we report a quantitative image analysis pipeline combined with mapping of molecular markers for mining >2,000,000 images and >1300 metagenomes from surface, deep chlorophyll maximum and mesopelagic seawater samples across 6 size fractions (<0.2-2000m). We use this approach to characterise the diversity, abundance, biovolume and distribution of symbiotic, colony-forming and particle-associated diazotrophs at a global scale. We show that imaging and PCR-free molecular data are congruent. Sequence reads indicate diazotrophs are detected from the ultrasmall bacterioplankton (<0.2m) to mesoplankton (180-2000 mu m) communities, while images predict numerous symbiotic and colony-forming diazotrophs (>20 mu m). Using imaging and molecular data, we estimate that polyploidy can substantially affect gene abundances of symbiotic versus colony-forming diazotrophs. Our results support the canonical view that larger diazotrophs (>10 mu m) dominate the tropical belts, while unicellular cyanobacterial and non-cyanobacterial diazotrophs are globally distributed in surface and mesopelagic layers. We describe co-occurring diazotrophic lineages of different lifestyles and identify high-density regions of diazotrophs in the global ocean. Overall, we provide an update of marine diazotroph biogeographical diversity and present a new bioimaging-bioinformatic workflow. Nitrogen fixation by diazotrophs is critical for marine primary production. Using Tara Oceans datasets, this study combines a quantitative image analysis pipeline with metagenomic mining to provide an improved global overview of diazotroph abundance, diversity and distribution.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Pierella Karlusi ...
-
Pelletier, Eric
-
Lombard, Fabien
-
Carsique, Madeli ...
-
Dvorak, Etienne
-
Colin, Sébastien
-
visa fler...
-
Picheral, Marc
-
Cornejo-Castillo ...
-
Acinas, Silvia G ...
-
Pepperkok, Raine ...
-
Karsenti, Eric
-
de Vargas, Colom ...
-
Wincker, Patrick
-
Bowler, Chris
-
Foster, Rachel A ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
- Artiklar i publikationen
-
Nature Communica ...
- Av lärosätet
-
Stockholms universitet