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Differential protei...
Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf-5
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McKew, Boyd A. (författare)
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Johnson, Richard (författare)
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Clothier, Lindsay (författare)
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Skeels, Karl (författare)
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Ross, Matthew S. (författare)
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Metodiev, Metodi (författare)
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Frenzel, Max (författare)
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Gieg, Lisa M. (författare)
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- Martin, Jonathan W. (författare)
- Stockholms universitet,Institutionen för miljövetenskap
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Hough, Michael A. (författare)
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Whitby, Corinne (författare)
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(creator_code:org_t)
- 2021-07-19
- 2021
- Engelska.
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Ingår i: MicrobiologyOpen. - : Wiley. - 2045-8827. ; 10:4
- Relaterad länk:
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https://doi.org/10.1...
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Naphthenic acids (NAs) are carboxylic acids with the formula (CnH2n+ZO2) and are among the most toxic, persistent constituents of oil sands process-affected waters (OSPW), produced during oil sands extraction. Currently, the proteins and mechanisms involved in NA biodegradation are unknown. Using LC-MS/MS shotgun proteomics, we identified proteins overexpressed during the growth of Pseudomonas fluorescens Pf-5 on a model NA (4 '-n-butylphenyl)-4-butanoic acid (n-BPBA) and commercial NA mixture (Acros). By day 11, >95% of n-BPBA was degraded. With Acros, a 17% reduction in intensity occurred with 10-18 carbon compounds of the Z family -2 to -14 (major NA species in this mixture). A total of 554 proteins (n-BPBA) and 631 proteins (Acros) were overexpressed during growth on NAs, including several transporters (e.g., ABC transporters), suggesting a cellular protective response from NA toxicity. Several proteins associated with fatty acid, lipid, and amino acid metabolism were also overexpressed, including acyl-CoA dehydrogenase and acyl-CoA thioesterase II, which catalyze part of the fatty acid beta-oxidation pathway. Indeed, multiple enzymes involved in the fatty acid oxidation pathway were upregulated. Given the presumed structural similarity between alkyl-carboxylic acid side chains and fatty acids, we postulate that P. fluorescens Pf-5 was using existing fatty acid catabolic pathways (among others) during NA degradation.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- naphthenic acids
- oil sands process-affected water
- proteomics
- Pseudomonas fluorescens
- tailing ponds
- toxicity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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McKew, Boyd A.
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Johnson, Richard
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Clothier, Lindsa ...
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Skeels, Karl
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Ross, Matthew S.
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Metodiev, Metodi
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visa fler...
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Frenzel, Max
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Gieg, Lisa M.
-
Martin, Jonathan ...
-
Hough, Michael A ...
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Whitby, Corinne
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Artiklar i publikationen
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MicrobiologyOpen
- Av lärosätet
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Stockholms universitet