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The Analysis of Yea...
The Analysis of Yeast Mitochondrial Translation
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- Carlström, Andreas (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Rzepka, Magdalena (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Ott, Martin (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- 2020-11-24
- 2021
- English.
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In: Mitochondrial Gene Expression. - New York : Humana Press. - 9781071608333 - 9781071608340 ; , s. 227-242
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Abstract
Subject headings
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- The mitochondrial genome encodes only a handful of proteins, but methods to track their synthesis are highly limited. Saccharomyces cerevisiae is a model organism that offers possibilities to expand the classical systems to analyze mitochondrial translation. In this chapter, we present two approaches of monitoring mitochondrial protein synthesis. Labeling of mitochondrially translated products with radioactive amino acids can be performed either in intact cells or in isolated mitochondria. However, these classical methods have disadvantages that can affect cell physiology and hence are not suitable for all types of research questions. Some of these limitations can be overcome by the use of reporter genes that are inserted into yeast genetic screens mitochondrial DNA via biolistic transformation. These reporter genes can be used for yeast genetic screen and to monitor regulation and efficiency of mitochondrial translation with a variety of methods.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Translation
- Mitochondria
- Yeast
- Reporter genes
- Protein synthesis
Publication and Content Type
- ref (subject category)
- kap (subject category)
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