In-situ sequencing of expressed mutations via RNA-templated gap filling of padlock probes.

• Detecting complex mutations in-situ within fixed cells or tissues is challenging, because of low sensitivity or poor specificity. We developed an efficient and specific molecular tool based on the RNA-templated and controlled gap-filling of padlock probes. Our method enables the accurate in-situ sequencing of unknown RNA stretches surrounded by known flanking regions, allowing the in-situ detection of expressed mutations with higher sensitivity and specificity than currently available tools.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

in situ hybridisation

in situ sequencing

spatial transcriptomics

biokemi

Biochemistry

Publikations- och innehållstyp

vet (ämneskategori)

ovr (ämneskategori)