Sökning: id:"swepub:oai:DiVA.org:su-25174" >
Characterization of...
Characterization of alkyl-cobalamins formed in trapping of epoxide metabolites of 1,3-butadiene
-
- Fred, Charlotta (författare)
- Stockholms universitet,Institutionen för miljökemi
-
Haglund, Johanna (författare)
-
Alsberg, Tomas (författare)
-
visa fler...
-
- Rydberg, Per (författare)
- Stockholms universitet,Avdelningen för miljökemi
-
- Minten, Johanna (författare)
- Stockholms universitet,Avdelningen för miljökemi
-
- Törnqvist, Margareta (författare)
- Stockholms universitet,Avdelningen för miljökemi
-
visa färre...
-
(creator_code:org_t)
- Wiley, 2004
- 2004
- Engelska.
-
Ingår i: Journal of Separation Science. - : Wiley. - 1615-9306 .- 1615-9314. ; 27:7-8, s. 607-612
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Analytical methods facilitating studies of electrophilically reactive and genotoxic compounds in vitro and in vivo are needed. The strong nucleophile, cob(I)alamin, formed by reduction of Vitamin B12 [cob(III)alamin], may be used for trapping and analysis of 1,2-epoxides and other electrophiles. In the present study, cob(I)alamin is evaluated as an analytical tool for 1,2-epoxide metabolites (oxiranes) of 1,3-butadiene. Products of reaction of cob(I)alamin with 1,2-epoxy-3-butene (EB), 1,2:3,4-diepoxybutane (DEB), and 1,2-epoxy-3,4-butanediol (EBdiol) have been analyzed by reversed phase high performance liquid chromatography (HPLC) coupled on-line to electrospray ionization mass spectrometry (ESI-MS) and ultraviolet diode array detection (UV-DAD). It was shown that a specific alkyl-Cbl complex is formed for each metabolite and that it was possible to discriminate between the products by HPLC-UV and by LC-MS. Quantification of DEB with the method by use of another 1,2-epoxide as an internal standard was successfully performed. The possibility of using cob(I)alamin for trapping and analysis of the three oxirane metabolites of 1,3-butadiene will facilitate quantitative comparisons of species in vitro with regard to metabolism of 1,3-butadiene.
Ämnesord
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
Nyckelord
- 1
- 3-Butadiene • Diepoxybutane • Cobalamin • Reactive metabolites • LC-MS
- Environmental chemistry
- Miljökemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas