Structural and Interaction Studies of Bacterial Polysaccharides by NMR Spectroscopy

• An introduction to bacterial polysaccharides and the methods for structural determination are described in the first two parts of the thesis.In a structural elucidation of bacterial polysaccharides NMR experiments are important as is component analysis. A short description of immunochemical methods such as enzyme immunoassays is included. Two NMR techniques used for interaction studies, trNOE and STD NMR, are also discussed. The third part of the thesis discusses and summarizes the results from the included papers. The structures of the exopolysaccharides produced by two lactic acid bacteria are determined by one- and two dimensional NMR experiments. One is a heteropolysaccharide produced by Streptococcus thermophilus and the other a homopolysaccharide produced by Propionibacterium freudenreichii. The structure of an acidic polysaccharide from a marine bacterium with two serine residues in the repeating unit is also investigated. The structural and immunological relationship between two O-antigenic polysaccharides from Escherichia coli strain 180/C3 and O5 is discussed and investigated. Finally, interaction studies of an octasaccharide derived from the Salmonella enteritidis O-antigen and a bacteriophage are described which were performed with NMR experiments.

Ämnesord

NATURVETENSKAP  -- Kemi -- Organisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Organic Chemistry (hsv//eng)

Nyckelord

bacterial polysaccharides

NMR

interaction studies

structural studies

Escherichia coli

Salmonella enteritidis

Streptococcus thermophilus

Propionibacterium freudenreichii

Pseudoalteromonas aliena

P22 TSP

Organic chemistry

Organisk kemi

Publikations- och innehållstyp

vet (ämneskategori)

dok (ämneskategori)