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Assembly of a fragm...
Assembly of a fragmented ribonucleotide reductase by protein interaction domains derived from a mobile genetic element
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- Crona, Mikael, 1981- (författare)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik,Britt-Marie Sjöberg
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- Sjöberg, Britt-Marie (författare)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Moffatt, Connor (författare)
- Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario
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- Edgell, David R. (författare)
- Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario
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- Friedrich, Nancy C. (författare)
- Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario
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- Hofer, Anders (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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(creator_code:org_t)
- 2010-10-22
- 2011
- Engelska.
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Ingår i: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 39:4, s. 1381-1389
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Ribonucleotide reductase (RNR) is a critical enzyme of nucleotide metabolism, synthesizing precursors for DNA replication and repair. In prokaryotic genomes, RNR genes are commonly targeted by mobile genetic elements, including free standing and intron-encoded homing endonucleases and inteins. Here, we describe a unique molecular solution to assemble a functional product from the RNR large subunit gene, nrdA that has been fragmented into two smaller genes by the insertion of mobE, a mobile endonuclease. We show that unique sequences that originated during the mobE insertion and that are present as C- and N-terminal tails on the split NrdA-a and NrdA-b polypeptides, are absolutely essential for enzymatic activity. Our data are consistent with the tails functioning as protein interaction domains to assemble the tetrameric (NrdA-a/NrdA-b)2 large subunit necessary for a functional RNR holoenzyme. The tails represent a solution distinct from RNA and protein splicing or programmed DNA rearrangements to restore function from a fragmented coding region and may represent a general mechanism to neutralize fragmentation of essential genes by mobile genetic elements.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Molecular biology
- Molekylärbiologi
- Molecular Biology
- molekylärbiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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