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Genome-Wide RNA Int...
Genome-Wide RNA Interference in Drosophila Cells Identifies G Protein-Coupled Receptor Kinase 2 as a Conserved Regulator of NF-kappa B Signaling
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- Valanne, Susanna (author)
- University of Tampere,Hultmark
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Myllymäki, Henna (author)
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Kallio, Jenni (author)
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- Schmid, Martin Rudolf, 1979- (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Hultmark
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- Kleino, Anni (author)
- University of Tampere,Hultmark
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Murumägi, Astrid (author)
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Airaksinen, Laura (author)
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Kotipelto, Tapio (author)
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Kaustio, Meri (author)
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Ulvila, Johanna (author)
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- Seyedoleslami Esfahani, Shiva (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Engström, Ylva (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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Silvennoinen, Olli (author)
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- Hultmark, Dan, 1949- (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Hultmark
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Parikka, Mataleena (author)
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Rämet, Mika (author)
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(creator_code:org_t)
- 2010-06-01
- 2010
- English.
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In: Journal of Immunology. - : The American Association of Immunologists. - 0022-1767 .- 1550-6606. ; 184:11, s. 6188-6198
- Related links:
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https://doi.org/10.4...
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Abstract
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- Because NF-kappa B signaling pathways are highly conserved in evolution, the fruit fly Drosophila melanogaster provides a good model to study these cascades. We carried out an RNA interference (RNAi)-based genome-wide in vitro reporter assay screen in Drosophila for components of NF-kappa B pathways. We analyzed 16,025 dsRNA-treatments and identified 10 novel NF-kappa B regulators. Of these, nine dsRNA-treatments affect primarily the Toll pathway. G protein-coupled receptor kinase (Gprk) 2, CG15737/Toll pathway activation mediating protein, and u-shaped were required for normal Drosomycin response in vivo. Interaction studies revealed that Gprk2 interacts with the Drosophila I kappa B homolog Cactus, but is not required in Cactus degradation, indicating a novel mechanism for NF-kappa B regulation. Morpholino silencing of the zebrafish ortholog of Gprk2 in fish embryos caused impaired cytokine expression after Escherichia coli infection, indicating a conserved role in NF-kappa B signaling. Moreover, small interfering RNA silencing of the human ortholog GRK5 in HeLa cells impaired NF-kappa B reporter activity. Gprk2 RNAi flies are susceptible to infection with Enterococcus faecalis and Gprk2 RNAi rescues Toll(10b)-induced blood cell activation in Drosophila larvae in vivo. We conclude that Gprk2/GRK5 has an evolutionarily conserved role in regulating NF-kappa B signaling. The Journal of Immunology, 2010, 184: 6188-6198.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Valanne, Susanna
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Myllymäki, Henna
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Kallio, Jenni
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Schmid, Martin R ...
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Kleino, Anni
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Murumägi, Astrid
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show more...
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Airaksinen, Laur ...
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Kotipelto, Tapio
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Kaustio, Meri
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Ulvila, Johanna
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Seyedoleslami Es ...
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Engström, Ylva
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Silvennoinen, Ol ...
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Hultmark, Dan, 1 ...
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Parikka, Matalee ...
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Rämet, Mika
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Journal of Immun ...
- By the university
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Stockholm University
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Uppsala University
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Umeå University