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NafA negatively con...
NafA negatively controls Neisseria meningitidis piliation
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- Kuwae, Asaomi (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Sjölinder, Hong (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Eriksson, Jens (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Eriksson, Sara (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Chen, Yao (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Jonsson, Ann-Beth (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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(creator_code:org_t)
- 2011-07-01
- 2011
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:7, s. e21749-
- Relaterad länk:
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https://doi.org/10.1...
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Bacterial auto-aggregation is a critical step during adhesion of N. meningitidis to host cells. The precise mechanisms and functions of bacterial auto-aggregation still remain to be fully elucidated. In this work, we characterize the role of a meningococcal hypothetical protein, NMB0995/NMC0982, and show that this protein, here denoted NafA, acts as an anti-aggregation factor. NafA was confirmed to be surface exposed and was found to be induced at a late stage of bacterial adherence to epithelial cells. A NafA deficient mutant was hyperpiliated and formed bundles of pili. Further, the mutant displayed increased adherence to epithelial cells when compared to the wild-type strain. In the absence of host cells, the NafA deficient mutant was more aggregative than the wild-type strain. The in vivo role of NafA in sepsis was studied in a murine model of meningococcal disease. Challenge with the NafA deficient mutant resulted in lower bacteremia levels and mortality when compared to the wild-type strain. The present study reveals that meningococcal NafA is an anti-aggregation factor with strong impact on the disease outcome. These data also suggest that appropriate bacterial auto-aggregation is controlled by both aggregation and anti-aggregation factors during Neisseria infection in vivo.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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