Search: id:"swepub:oai:DiVA.org:su-82120" >
Application of spli...
Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli
-
- Toddo, Stephen (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Söderström, Bill (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Palombo, Isolde, 1984- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
show more...
-
- von Heijne, Gunnar (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
-
- Norholm, Morten H. H. (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Daley, Daniel O. (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
show less...
-
(creator_code:org_t)
- 2012-08-21
- 2012
- English.
-
In: Protein Science. - : Wiley. - 0961-8368 .- 1469-896X. ; 21:10, s. 1571-1576
- Related links:
-
https://onlinelibrar...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- A topology map of a membrane protein defines the location of transmembrane helices and the orientation of soluble domains relative to the membrane. In the absence of a high-resolution structure, a topology map is an essential guide for studying structurefunction relationships. Although these maps can be predicted directly from amino acid sequence, the predictions are more accurate if combined with experimental data, which are usually obtained by fusing a reporter protein to the C-terminus of the protein. However, as reporter proteins are large, they cannot be used to report on the cytoplasmic/periplasmic location of the N-terminus of a protein. Here, we show that the bimolecular split-green fluorescent protein complementation system can overcome this limitation and can be used to determine the location of both the N- and C-termini of inner membrane proteins in Escherichia coli.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- topology
- membrane protein
- split-GFP
- inner membrane
- Biochemistry
- biokemi
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database