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A Membrane-Bound NA...
A Membrane-Bound NAC Transcription Factor, ANAC017, Mediates Mitochondrial Retrograde Signaling in Arabidopsis
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Ng, Sophia (författare)
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Ivanova, Aneta (författare)
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Duncan, Owen (författare)
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Law, Simon R. (författare)
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Van Aken, Olivier (författare)
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De Clercq, Inge (författare)
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Wang, Yan (författare)
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Carrie, Chris (författare)
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Xu, Lin (författare)
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- Kmiec, Beata (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Walker, Hayden (författare)
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Van Breusegem, Frank (författare)
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Whelan, James (författare)
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Giraud, Estelle (författare)
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(creator_code:org_t)
- 2013-09-17
- 2013
- Engelska.
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Ingår i: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 25:9, s. 3450-3471
- Relaterad länk:
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https://doi.org/10.1...
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http://www.plantcell...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Plants require daily coordinated regulation of energy metabolism for optimal growth and survival and therefore need to integrate cellular responses with both mitochondrial and plastid retrograde signaling. Using a forward genetic screen to characterize regulators of alternative oxidase1a (rao) mutants, we identified RAO2/Arabidopsis NAC domain-containing protein17 (ANAC017) as a direct positive regulator of AOX1a. RAO2/ANAC017 is targeted to connections and junctions in the endoplasmic reticulum (ER) and F-actin via a C-terminal transmembrane (TM) domain. A consensus rhomboid protease cleavage site is present in ANAC017 just prior to the predicted TM domain. Furthermore, addition of the rhomboid protease inhibitor N-p-Tosyl-L-Phe chloromethyl abolishes the induction of AOX1a upon antimycin A treatment. Simultaneous fluorescent tagging of ANAC017 with N-terminal red fluorescent protein (RFP) and C-terminal green fluorescent protein (GFP) revealed that the N-terminal RFP domain migrated into the nucleus, while the C-terminal GFP tag remained in the ER. Genome-wide analysis of the transcriptional network regulated by RAO2/ANAC017 under stress treatment revealed that RAO2/ANAC017 function was necessary for > 85% of the changes observed as a primary response to cytosolic hydrogen peroxide (H2O2), but only; 33% of transcriptional changes observed in response to antimycin A treatment. Plants with mutated rao2/anac017 were more stress sensitive, whereas a gain-of-function mutation resulted in plants that had lower cellular levels of H2O2 under untreated conditions.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Ng, Sophia
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Ivanova, Aneta
-
Duncan, Owen
-
Law, Simon R.
-
Van Aken, Olivie ...
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De Clercq, Inge
-
visa fler...
-
Wang, Yan
-
Carrie, Chris
-
Xu, Lin
-
Kmiec, Beata
-
Walker, Hayden
-
Van Breusegem, F ...
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Whelan, James
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Giraud, Estelle
-
visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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The Plant Cell
- Av lärosätet
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Stockholms universitet