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TiO2 coating promot...
TiO2 coating promotes human mesenchymal stem cell proliferation without the loss of their capacity for chondrogenic differentiation
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- Kaitainen, Salla (författare)
- Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
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- Mähönen, Anssi (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland
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- Lappalainen, Reijo (författare)
- Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
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- Kröger, Heikki (författare)
- Department of Orthopaedics and Traumatology, Kuopio University Hospital, Kuopio, Finland
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- Lammi, Mikko, 1961- (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Qu, Chengjuan, 1967- (författare)
- Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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(creator_code:org_t)
- 2013-04-17
- 2013
- Engelska.
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Ingår i: Biofabrication. - : Institute of Physics (IOP). - 1758-5090 .- 1758-5082. ; 5:2, s. 025009-
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Human mesenchymal stem cells (hMSCs) are used in applications, which may require a large amount of cells; therefore, efficient expansion of the cells is desired. We studied whether TiO2 coating on plastic cell culture dishes could promote proliferation of hMSCs without adverse effects in chondrogenic differentiation. TiO2-films were deposited on polystyrene dishes and glass coverslips using an ultrashort pulsed laser deposition technique. Human MSCs from three donors were expanded on them until 95% confluence, and the cells were evaluated by morphology, immunocytochemistry and quantitative RT-PCR (qRT-PCR). The chondrogenic differentiation in pellets was performed after cultivation on TiO2-coated dishes. Chondrogenesis was evaluated by histological staining of proteoglycans and type II collagen, and qRT-PCR. Human MSC-associated markers STRO-1, CD44, CD90 and CD146 did not change after expansion on TiO2-coated coverslips. However, the cell number after a 48h-culture period was significantly higher on TiO2-coated culture dishes. Importantly, TiO2 coating caused no significant differences in the proteoglycan and type II collagen staining of the pellets, or the expression of chondrocyte-specific genes in the chondrogenesis assay. Thus, the proliferation of hMSCs could be significantly increased when cultured on TiO2-coated dishes without weakening their chondrogenic differentiation capacity. The transparency of TiO2-films allows easy monitoring of the cell growth and morphology under a phase-contrast microscope.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Annan materialteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Other Materials Engineering (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- stem cell biology
- biomaterials
- thin films
- laser deposition
- tissue engineeering
- growth and division
- biological physics
- biologisk kemi
- biological chemistry
- cell research
- cellforskning
- Materials Science
- materialvetenskap
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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