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Five percent oxygen...
Five percent oxygen tension is not beneficial for neocartilage formation in scaffold-free cell cultures
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- Qu, Chengjuan, 1967- (författare)
- Department of Biosciences, Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Lindeberg, Heli (författare)
- Department of Biosciences, University of Eastern Finland, Kuopio, Finland
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- Ylärinne, Janne, 1990- (författare)
- Department of Biosciences, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Lammi, Mikko, 1961- (författare)
- Department of Biosciences, Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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(creator_code:org_t)
- 2012-03-06
- 2012
- Engelska.
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Ingår i: Cell and Tissue Research. - : Springer Publishing Company. - 0302-766X .- 1432-0878. ; 348:1, s. 109-117
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We have investigated whether 5% oxygen tension (O(2)) is beneficial for neocartilage formation when chondrocytes are cultured in transwell-COL inserts. Six million bovine primary chondrocytes were cultured in an insert with DMEM supplemented with 10% fetal bovine serum and antibiotics, with or without glucosamine sulphate (GS) in a 5% or 20% O(2) environment for 2, 4, or 6 weeks. The samples were collected for the histological staining of proteoglycans (PGs) and type II collagen, quantitative reverse transcription with the polymerase chain reaction (RT-PCR) analyses of the mRNA expression of aggrecan and procollagen α(1)(II), procollagen α(2)(I) and hyaluronan synthase 2, quantitation of PGs, and agarose gel electrophoresis. Neocartilage produced at 20% O(2) appeared larger than that at 5% O(2). Histological staining showed that more PGs and type II collagen and better native cartilage structure were produced at 20% than at 5% O(2). The thickness of neocartilage increased during the culture period. Quantitative RT-PCR showed that the procollagen α(1)(II) mRNA expression level was significantly higher at 20% than at 5% O(2). However, no significant difference in gene expression and PG content was found between control and GS-treated cultures at either 20% or 5% O(2). Thus, in contrast to monolayer cultures, engineered cartilage from scaffold-free cultured chondrocytes at 20% O(2) produced better extracellular matrix (ECM) than that at 5% O(2). PGs were mainly large. Exogenous GS was not beneficial for the ECM in scaffold-free chondrocyte cultures.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
Nyckelord
- Primary chondrocyte
- Tissue engineering
- Oxygen tension
- Glucosamine sulphate
- Cell culture
- Neocartilage
- biokemi
- Biochemistry
- cell research
- cellforskning
- ortopedi
- Orthopaedics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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