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Hypotonic challenge...
Hypotonic challenge modulates cell volumes differently in the superficial zone of intact articular cartilage and cartilage explant.
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Turunen, Siru (författare)
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- Lammi, Mikko, 1961- (författare)
- Department of Biosciences, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Saarakkala, Simo (författare)
- Department of Applied Physics, University of Eastern Finland, Kuopio, Finland; Department of Diagnostic Radiology, University of Oulu and Oulu University Hospital, Oulu, Finland
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- Koistinen, Arto (författare)
- Department of Applied Physics, University of Eastern Finland, Kuopio, Finland; BioMater Centre, University of Eastern Finland, Kuopio, Finland
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- Korhonen, Rami (författare)
- Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
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(creator_code:org_t)
- 2011-08-30
- 2012
- Engelska.
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Ingår i: Biomechanics and Modeling in Mechanobiology. - : Springer. - 1617-7959 .- 1617-7940. ; 11:5, s. 665-75
- Relaterad länk:
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http://link.springer...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The objective of this study was to evaluate the effect of sample preparation on the biomechanical behaviour of chondrocytes. We compared the volumetric and dimensional changes of chondrocytes in the superficial zone (SZ) of intact articular cartilage and cartilage explant before and after a hypotonic challenge. Calcein-AM labelled SZ chondrocytes were imaged with confocal laser scanning microscopy through intact cartilage surfaces and through cut surfaces of cartilage explants. In order to clarify the effect of tissue composition on cell volume changes, Fourier Transform Infrared microspectroscopy was used for estimating the proteoglycan and collagen contents of the samples. In the isotonic medium (300 mOsm), there was a significant difference (p < 0.05) in the SZ cell volumes and aspect ratios between intact cartilage samples and cartilage explants. Changes in cell volumes at both short-term (2 min) and long-term (2 h) time points after the hypotonic challenge (180 mOsm) were significantly different (p < 0.05) between the groups. Further, proteoglycan content was found to correlate significantly (r(2) = 0.63, p < 0.05) with the cell volume changes in cartilage samples with intact surfaces. Collagen content did not correlate with cell volume changes. The results suggest that the biomechanical behaviour of chondrocytes following osmotic challenge is different in intact cartilage and in cartilage explant. This indicates that the mechanobiological responses of cartilage and cell signalling may be significantly dependent on the integrity of the mechanical environment of chondrocytes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Nyckelord
- Articular cartilage
- Chondrocyte
- Mechanobiology
- Fluorescence microscopy
- Hypotonic
- Osmotic pressure
- cell research
- cellforskning
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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