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Proteomic changes i...
Proteomic changes in articular cartilage of human endemic osteoarthritis in China.
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- Ma, Wei-Juan (författare)
- Medical College of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Xi'an, Shaanxi, China
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- Guo, Xiong (författare)
- Medical College of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Xi'an, Shaanxi, China
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- Liu, Jiang-Tao (författare)
- Medical College of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Xi'an, Shaanxi, China
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- Liu, Rui-Yu (författare)
- Medical College of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Xi'an, Shaanxi, China
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- Hu, Jian-Wen (författare)
- Research Center for Proteome Analysis, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
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- Sun, An-Guo (författare)
- Research Center for Proteome Analysis, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
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- Yu, Yue-Xiang (författare)
- Shaanxi Provincial Institute For Endemic Disease Control, Xi'an, Shaanxi, China
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- Lammi, Mikko, 1961- (författare)
- Department of Biosciences, University of Eastern Finland, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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(creator_code:org_t)
- 2011-06-17
- 2011
- Engelska.
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Ingår i: Proteomics. - : Wiley-VCH Verlagsgesellschaft. - 1615-9853 .- 1615-9861. ; 11:14, s. 2881-90
- Relaterad länk:
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http://onlinelibrary...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Kashin-Beck disease (KBD) is a chronic endemic osteochondropathy with unclear pathogenesis. It is a degenerative disease similar to osteoarthritis, but with different manifestations of cartilage damage. The aim of this investigation was to show the protein changes in KBD cartilage and to identify the candidate proteins in order to understand the pathogenesis of the disease. Proteins were extracted from the media of primary cell cultures of KBD and normal chondrocytes, and separated by two-dimensional fluorescence difference gel electrophoresis (2-D DIGE). MALDI-TOF/TOF analysis revealed statistically significant differences in 27 proteins from KBD chondrocyte cultures, which consisted of 17 up-regulated and ten down-regulated proteins. The results were further validated by Western blot analysis. The proteins identified are mainly involved in cellular redox homeostasis and stress response (MnSOD, Hsp27, Peroxiredoxin-1, and Cofilin-1), glycolysis (PGK-1, PGM-1, α-enolase), and cell motility and cytoskeletal organization (Actin, Calponin-2, and Keratin). These KBD-associated proteins indicate that cytoskeletal remodeling, glycometabolism, and oxidative stress are abnormal in KBD articular cartilage.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- biokemi
- Biochemistry
- ortopedi
- Orthopaedics
- cell research
- cellforskning
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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