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Recombinant human t...
Recombinant human type II collagen as a material for cartilage tissue engineering.
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- Pulkkinen, Hertta (författare)
- Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland
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- Tiitu, Virpi (författare)
- Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland
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- Valonen, Piia (författare)
- Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland
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- Hämäläinen, Eija-Riitta (författare)
- Bioprocess Engineering Laboratory, University of Oulu, Oulu, Finland
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- Lammi, Mikko, 1961- (författare)
- Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland; Department of Biosciences, Applied Biotechnology, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Kiviranta, Ilkka (författare)
- Department of Orthopaedics and Traumatology, Jyväskylä Central Hospital, Jyväskylä, Finland; Department of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland
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(creator_code:org_t)
- Wichtig Editore Srl, 2008
- 2008
- Engelska.
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Ingår i: International Journal of Artificial Organs. - : Wichtig Editore Srl. - 0391-3988 .- 1724-6040. ; 31:11, s. 960-969
- Relaterad länk:
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http://www.artificia...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- PURPOSE: Collagen type II is the major component of cartilage and would be an optimal scaffold material for reconstruction of injured cartilage tissue. In this study, the feasibility of recombinant human type II collagen gel as a 3-dimensional culture system for bovine chondrocytes was evaluated in vitro.METHODS: Bovine chondrocytes (4x106 cells) were seeded within collagen gels and cultivated for up to 4 weeks. The gels were investigated with confocal microscopy, histology, and biochemical assays.RESULTS: Confocal microscopy revealed that the cells maintained their viability during the entire cultivation period. The chondrocytes were evenly distributed inside the gels, and the number of cells and the amount of the extracellular matrix increased during cultivation. The chondrocytes maintained their round phenotype during the 4-week cultivation period. The glycosaminoglycan levels of the tissue increased during the experiment. The relative levels of aggrecan and type II collagen mRNA measured with realtime polymerase chain reaction (PCR) showed an increase at 1 week.CONCLUSION: Our results imply that recombinant human type II collagen is a promising biomaterial for cartilage tissue engineering, allowing homogeneous distribution in the gel and biosynthesis of extracellular matrix components.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Nyckelord
- Chondrocyte
- recombinant type II collagen
- tissue engineering
- proteoglycans
- cell research
- cellforskning
- biokemi
- Biochemistry
- ortopedi
- Orthopaedics
- Materials Science
- materialvetenskap
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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