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Geldanamycin increases 4-hydroxynonenal (HNE)-induced cell death in human retinal pigment epithelial cells.
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- Kaarniranta, Kai (författare)
- Department of Ophthalmology, University of Kuopio, Kuopio, Finland; Department of Ophthalmology, Kuopio University Hospital, Kuopio, Finland
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- Ryhänen, Tuomas (författare)
- Department of Ophthalmology, University of Kuopio, Kuopio, Finland
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- Karjalainen, Hannu (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Lammi, Mikko, 1961- (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Suuronen, Tiina (författare)
- Department of Neuroscience and Neurology, University of Kuopio, Kuopio, Finland
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- Huhtala, Anne (författare)
- University of Tampere Medical School, Tampere, Finland
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- Kontkanen, Matti (författare)
- Department of Ophthalmology, North Karelia Central Hospital, Joensuu, Finland
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- Teräsvirta, Markku (författare)
- Department of Ophthalmology, Kuopio University Hospital, Kuopio, Finland
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- Uusitalo, Hannu (författare)
- Department of Ophthalmology, University of Kuopio, Kuopio, Finland
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- Salminen, Antero (författare)
- Department of Neuroscience and Neurology, University of Kuopio, Kuopio, Finland; Department of Neurology, Kuopio University Hospital, Kuopio, Finland
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(creator_code:org_t)
- Elsevier, 2005
- Engelska.
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Ingår i: Neuroscience Letters. - : Elsevier. - 0304-3940 .- 1872-7972. ; 382:1-2, s. 185-190
- Relaterad länk:
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http://www.sciencedi...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Development of age-related macular degeneration (AMD) is associated with functional abnormalities and cell death in retinal pigment epithelial (RPE) cells attributable to oxidative stress. To minimize the adverse effects of oxidative stress, cells activate their defence systems, e.g., via increased expression of heat shock protein (Hsp), activation of stress sensitive AP-1 and NF-kappaB transcription factors. In this study, we examined the accumulation of Hsp70 protein, activation of AP-1 and NF-kappaB transcription factors in human ARPE-19 cells subjected to a 4-hydroxynonenal (HNE)-induced oxidative stress. In addition, the influence of Hsp90 inhibitor geldanamycin (GA) was studied in HNE-treated cells. Mitochondrial metabolic activity and apoptosis were determined to evaluate cell death in the ARPE-19 cells. The ARPE-19 cells showed increased accumulation of Hsp70 protein before of the cytotoxic hallmarks appearing in response to HNE. In contrast, increased DNA-binding activities of AP-1 or NF-kappaB transcription factors were not seen under HNE insults. Interestingly, GA significantly increased cell death in the HNE-treated cells, which was involved in caspase-3 independent apoptosis. This study reveals that the Hsps have an important role in the cytoprotection of RPE cells subjected to HNE-derived oxidative stress.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
Nyckelord
- Opthalmology
- retinal pigment epithelial cells
- hydrynonenal
- cel death
- cell research
- cellforskning
- oftalmiatrik
- Ophtalmology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
- Av författaren/redakt...
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Kaarniranta, Kai
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Ryhänen, Tuomas
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Karjalainen, Han ...
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Lammi, Mikko, 19 ...
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Suuronen, Tiina
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Huhtala, Anne
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visa fler...
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Kontkanen, Matti
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Teräsvirta, Mark ...
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Uusitalo, Hannu
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Salminen, Antero
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Oftalmologi
- Artiklar i publikationen
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Neuroscience Let ...
- Av lärosätet
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Umeå universitet