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Elevated protein co...
Elevated protein content and prolyl 4-hydroxylase activity in severely degenerated human annulus fibrosus.
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- Kääpä, Eeva (författare)
- Department of Physical, Medicine and Rehabilitation, Helsinki University Hospital, Helsinki, Finland
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- Wang, Wei (författare)
- Department of Biology of Physical Activity, University of Jyväskylä, Finland
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- Takala, Timo (författare)
- Department of Biology of Physical Activity, University of Jyväskylä, Finland
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- Poussa, Mikko (författare)
- Orthopaedic Hospital of the Invalid Foundation, Helsinki, Finland
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- Konttinen, Yrjö (författare)
- Fourth Department of Medicine, Helsinki University Hospital, Helsinki, Finland; Department of Biology of Physical Activity, University of Jyväskylä, Finland
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- Lammi, Mikko, 1961- (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Vanharanta, Heikki (författare)
- Department of Physical, Medicine and Rehabilitation, Oulu University, Finland
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(creator_code:org_t)
- Informa Healthcare, 2000
- 2000
- Engelska.
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Ingår i: Connective Tissue Research. - : Informa Healthcare. - 0300-8207 .- 1607-8438. ; 41:2, s. 93-99
- Relaterad länk:
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http://informahealth...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Alterations involved with the intervertebral disc degeneration are partly well described, however, it is not so well known how collagen network is affected by the disease. We analyzed the rate of collagen biosynthesis (estimated by the enzymic activities of prolyl 4-hydroxylase and galactosylhydroxylysyl glucosyltransferase) and the level of hydroxylysylpyridinoline and lysylpyridinoline crosslinks both in normal (n=7) and degenerated (n=7) human annulus fibrosus. The activity of prolyl 4-hydroxylase was significantly increased in degenerated tissue. However, no significant changes in the collagen content or in the amount of hydroxylysylpyridinoline and lysylpyridinoline collagen crosslinks were observed. On the other hand, the content of soluble proteins was significantly increased. Our results suggest that collagen biosynthesis is increased in degenerated human annulus fibrosus, obviously to compensate the impairment of collagen fibers. The faster turnover of collagen in degenerated annulus fibrosus, suggested by the increased prolyl 4-hydroxylase activity and unchanged collagen content, seems not to cause any significant changes in its mature pyridinium crosslink concentrations.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Human
- intervertbral disc
- degeneration
- annulus fibrosus
- collagen
- prolyl 4-hydroxylase
- biokemi
- Biochemistry
- cell research
- cellforskning
- ortopedi
- Orthopaedics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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