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Coordinated regulat...
Coordinated regulation of hyaluronan and aggrecan content in the articular cartilage of immobilized and exercised dogs.
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- Haapala, Jussi (författare)
- Department of Surgery, Kuopio University Hospital, Kuopio, Finland
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- Lammi, Mikko, 1961- (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group
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- Inkinen, Ritva (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Parkkinen, Jyrki (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Ågren, Ulla (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Arokoski, Jari (författare)
- Department of Physical and Rehabilitation Medicine, Kuopio University Hospital, Kuopio, Finland
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- Kiviranta, Ilkka (författare)
- Department of Surgery, Kuopio University Hospital, Kuopio, Finland
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- Helminen, Heikki (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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- Tammi, Markku (författare)
- Department of Anatomy, University of Kuopio, Kuopio, Finland
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(creator_code:org_t)
- Journal of Rheumatology, 1996
- 1996
- Engelska.
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Ingår i: Journal of Rheumatology. - : Journal of Rheumatology. - 0315-162X .- 1499-2752. ; 23:9, s. 1586-1593
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- OBJECTIVE: To study the influence of joint loading and immobilization on articular cartilage hyaluronan concentration and histological distribution in the knee joints of young dogs subjected to 11 weeks' immobilization by splinting, and 15 weeks' running exercise at a rate of 40 km/day.METHODS: The amount of hyaluronan in articular cartilage was determined by a competitive binding assay using a biotinylated hyaluronan binding complex (HABC) of aggrecan and link protein. Histologic sections were stained for the localization of hyaluronan with the HABC probe. Extracted proteoglycans were characterized by sodium dodecyl sulfate agarose gel electrophoresis.RESULTS: Immobilization significantly reduced the concentration of hyaluronan in all sites studied (tibial and femoral condyles, patellar surface of femur). The proportion of hyaluronan to total uronic acid (mainly from aggrecan) remained unchanged because of a concurrent decrease in aggrecan. The ratio of hyaluronan and aggrecan remained constant also in runners. The staining pattern of free hyaluronan in the tissue sections and the electrophoretic mobility of the extracted proteoglycans were not affected by the different loading regimes.CONCLUSION: Reduced joint loading due to splint immobilization significantly decreases both hyaluronan and aggrecan in the articular cartilage. The remarkably parallel changes in aggrecan and hyaluronan content suggest that joint loading exerts a coordinated influence on their metabolism.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Articular cartilage
- immobilization
- exercise
- hyaluronan
- proteogylcans
- dog
- biokemi
- Biochemistry
- cell research
- cellforskning
- ortopedi
- Orthopaedics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Haapala, Jussi
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Lammi, Mikko, 19 ...
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Inkinen, Ritva
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Parkkinen, Jyrki
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Ågren, Ulla
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Arokoski, Jari
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visa fler...
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Kiviranta, Ilkka
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Helminen, Heikki
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Tammi, Markku
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Ortopedi
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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Journal of Rheum ...
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