Proteoglycans in the intervertebral disc of young dogs following strenuous running exercise.

• The proteoglycans (PGs) of intervertebral disc were studied in ten beagles which ran on a treadmill for one year (up to 40 km/day) and in ten non-running control dogs. Nucleus pulposus and annulus fibrosus from cervical (C5) and thoracic (T6 and T12) discs were labeled in vitro with 35SO4. The extractability, concentration and synthesis of PGs, and the electrophoretic subpopulations, aggregation and glycosaminoglycan chain lengths of newly-synthesized and total PGs were measured. Sulfate incorporation was significantly elevated by running in the C5 disc and reduced in the annulus of T6 discs. In the annulus of the T6 discs the concentration of total PGs was significantly lower although that of dermatan sulfate PGs was actually higher than in the controls. The results show that enhanced loading of the spine exerts significant alterations in the intervertebral disc PGs in a spine-level specific manner. In the most strained area of the spine (upper thoracic), the alterations in the runners suggest compromised biomechanical properties of the disc.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Ortopedi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Orthopaedics (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Intervertebral disc

exercise

proteoglycan

dog

biokemi

Biochemistry

ortopedi

Orthopaedics

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