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Comprehensive metab...
Comprehensive metabolomics analysis of prostate cancer tissue in relation to tumor aggressiveness and TMPRSS2-ERG fusion status
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- Dudka, Ilona (författare)
- Umeå universitet,Kemiska institutionen
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- Thysell, Elin (författare)
- Umeå universitet,Patologi
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- Lundquist, Kristina (författare)
- Umeå universitet,Kemiska institutionen
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- Antti, Henrik, 1970- (författare)
- Umeå universitet,Kemiska institutionen
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Iglesias-Gato, Diego (författare)
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Flores-Morales, Amilcar (författare)
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- Bergh, Anders (författare)
- Umeå universitet,Patologi
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- Wikström, Pernilla (författare)
- Umeå universitet,Patologi
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- Gröbner, Gerhard (författare)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- 2020-05-18
- 2020
- Engelska.
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Ingår i: BMC Cancer. - : BioMed Central. - 1471-2407. ; 20:1
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://bmccancer.bi...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Prostate cancer (PC) can display very heterogeneous phenotypes ranging from indolent asymptomatic to aggressive lethal forms. Understanding how these PC subtypes vary in their striving for energy and anabolic molecules is of fundamental importance for developing more effective therapies and diagnostics. Here, we carried out an extensive analysis of prostate tissue samples to reveal metabolic alterations during PC development and disease progression and furthermore between TMPRSS2-ERG rearrangement-positive and -negative PC subclasses.Methods: Comprehensive metabolomics analysis of prostate tissue samples was performed by non-destructive high-resolution magic angle spinning nuclear magnetic resonance (H-1 HR MAS NMR). Subsequently, samples underwent moderate extraction, leaving tissue morphology intact for histopathological characterization. Metabolites in tissue extracts were identified by H-1/P-31 NMR and liquid chromatography-mass spectrometry (LC-MS). These metabolomics profiles were analyzed by chemometric tools and the outcome was further validated using proteomic data from a separate sample cohort.Results: The obtained metabolite patterns significantly differed between PC and benign tissue and between samples with high and low Gleason score (GS). Five key metabolites (phosphocholine, glutamate, hypoxanthine, arginine and alpha-glucose) were identified, who were sufficient to differentiate between cancer and benign tissue and between high to low GS. In ERG-positive PC, the analysis revealed several acylcarnitines among the increased metabolites together with decreased levels of proteins involved in beta-oxidation; indicating decreased acyl-CoAs oxidation in ERG-positive tumors. The ERG-positive group also showed increased levels of metabolites and proteins involved in purine catabolism; a potential sign of increased DNA damage and oxidative stress.Conclusions: Our comprehensive metabolomic analysis strongly indicates that ERG-positive PC and ERG-negative PC should be considered as different subtypes of PC; a fact requiring different, sub-type specific treatment strategies for affected patients.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Metabolomics
- Prostate cancer
- TMPRSS2-ERG
- H-1 HRMAS NMR
- Gleason score
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Dudka, Ilona
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Thysell, Elin
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Lundquist, Krist ...
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Antti, Henrik, 1 ...
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Iglesias-Gato, D ...
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Flores-Morales, ...
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visa fler...
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Bergh, Anders
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Wikström, Pernil ...
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Gröbner, Gerhard
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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BMC Cancer
- Av lärosätet
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Umeå universitet