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CAM-Delam: an in vi...
CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells
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- Panaliappan, Tamilarasan K. (författare)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Lena Gunhaga
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- Slekiene, Lina (författare)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Jonasson, Anna-Karin (författare)
- Umeå universitet,Institutionen för farmakologi och klinisk neurovetenskap
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- Gilthorpe, Jonathan D. (författare)
- Umeå universitet,Klinisk neurovetenskap
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- Gunhaga, Lena (författare)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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(creator_code:org_t)
- 2020-06-26
- 2020
- Engelska.
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 10:1
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The development of metastases is the major cause of cancer related death. To develop a standardized method that define the ability of human cancer cells to degrade the basement membrane, e.g. the delamination capacity, is of importance to assess metastatic aggressiveness. We now present the in vivo CAM-Delam assay to visualize and quantify the ability of human cancer cells to delaminate and invade. The method includes seeding cancer cells on the chick chorioallantoic membrane (CAM), followed by the evaluation of cancer-induced delamination and potential invasion within hours to a few days. By testing a range of human cancer cell lines in the CAM-Delam assay, our results show that the delamination capacity can be divided into four categories and used to quantify metastatic aggressiveness. Our results emphasize the usefulness of this assay for quantifying delamination capacity as a measurement of metastatic aggressiveness, and in unraveling the molecular mechanisms that regulate delamination, invasion, formation of micro-metastases and modulations of the tumor microenvironment. This method will be useful in both the preclinical and clinical characterization of tumor biopsies, and in the validation of compounds that may improve survival in metastatic cancer.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- biologisk beroendeforskning
- Biological Research on Drug Dependence
- Oncology
- onkologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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