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The maturase HydF e...
The maturase HydF enables [FeFe] hydrogenase assembly via transient, cofactor-dependent interactions
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- Németh, Brigitta (författare)
- Uppsala universitet,Molekylär biomimetik
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- Land, Henrik (författare)
- Uppsala universitet,Molekylär biomimetik
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- Magnuson, Ann (författare)
- Uppsala universitet,Molekylär biomimetik
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- Hofer, Anders (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå university
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- Berggren, Gustav (författare)
- Uppsala universitet,Molekylär biomimetik
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(creator_code:org_t)
- American Society for Biochemistry and Molecular Biology, 2020
- 2020
- Engelska.
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Ingår i: Journal of Biological Chemistry. - : American Society for Biochemistry and Molecular Biology. - 0021-9258 .- 1083-351X. ; 295:33, s. 11891-11901
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Abstract
Ämnesord
Stäng
- [FeFe] hydrogenases have attracted extensive attention in the field of renewable energy research because of their remarkable efficiency for H(2)gas production. H(2)formation is catalyzed by a biologically unique hexanuclear iron cofactor denoted the H-cluster. The assembly of this cofactor requires a dedicated maturation machinery including HydF, a multidomain [4Fe4S] cluster protein with GTPase activity. HydF is responsible for harboring and delivering a precatalyst to the apo-hydrogenase, but the details of this process are not well understood. Here, we utilize gas-phase electrophoretic macromolecule analysis to show that a HydF dimer forms a transient interaction complex with the hydrogenase and that the formation of this complex depends on the cofactor content on HydF. Moreover, Fourier transform infrared, electron paramagnetic resonance, and UV-visible spectroscopy studies of mutants of HydF show that the isolated iron-sulfur cluster domain retains the capacity for binding the precatalyst in a reversible fashion and is capable of activating apo-hydrogenase inin vitroassays. These results demonstrate the central role of the iron-sulfur cluster domain of HydF in the final stages of H-cluster assembly,i.e.in binding and delivering the precatalyst.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- metalloenzyme
- metal ion-protein interaction
- mass spectrometry (MS)
- Fourier transform IR (FTIR)
- hydrogenase
- cofactor
- chaperone
- scaffold
- iron-sulfur protein
- protein-protein interaction
- metallo-cofactor assembly
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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