Sökning: id:"swepub:oai:DiVA.org:umu-176105" >
Thrombospondin-1/CD...
Thrombospondin-1/CD47 signaling modulates transmembrane cation conductance, survival, and deformability of human red blood cells
-
Bissinger, Rosi (författare)
-
Petkova-Kirova, Polina (författare)
-
Mykhailova, Olga (författare)
-
visa fler...
-
- Oldenborg, Per-Arne (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
-
- Novikova, Elena (författare)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
-
Donkor, David A. (författare)
-
Dietz, Thomas (författare)
-
Bhuyan, Abdulla Al Mamun (författare)
-
Sheffield, William P. (författare)
-
Grau, Marijke (författare)
-
Artunc, Ferruh (författare)
-
Kaestner, Lars (författare)
-
Acker, Jason P. (författare)
-
Qadri, Syed M. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2020-09-18
- 2020
- Engelska.
-
Ingår i: Cell Communication and Signaling. - : Springer Nature. - 1478-811X. ; 18:1
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://umu.diva-por... (primary) (Raw object)
-
https://biosignaling...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Thrombospondin-1 (TSP-1), a Ca2+-binding trimeric glycoprotein secreted by multiple cell types, has been implicated in the pathophysiology of several clinical conditions. Signaling involving TSP-1, through its cognate receptor CD47, orchestrates a wide array of cellular functions including cytoskeletal organization, migration, cell-cell interaction, cell proliferation, autophagy, and apoptosis. In the present study, we investigated the impact of TSP-1/CD47 signaling on Ca2+ dynamics, survival, and deformability of human red blood cells (RBCs).Methods: Whole-cell patch-clamp was employed to examine transmembrane cation conductance. RBC intracellular Ca2+ levels and multiple indices of RBC cell death were determined using cytofluorometry analysis. RBC morphology and microvesiculation were examined using imaging flow cytometry. RBC deformability was measured using laser-assisted optical rotational cell analyzer.Results: Exposure of RBCs to recombinant human TSP-1 significantly increased RBC intracellular Ca2+ levels. As judged by electrophysiology experiments, TSP-1 treatment elicited an amiloride-sensitive inward current alluding to a possible Ca2+ influx via non-selective cation channels. Exogenous TSP-1 promoted microparticle shedding as well as enhancing Ca2+- and nitric oxide-mediated RBC cell death. Monoclonal (mouse IgG1) antibody-mediated CD47 ligation using 1F7 recapitulated the cell death-inducing effects of TSP-1. Furthermore, TSP-1 treatment altered RBC cell shape and stiffness (maximum elongation index).Conclusions: Taken together, our data unravel a new role for TSP-1/CD47 signaling in mediating Ca2+ influx into RBCs, a mechanism potentially contributing to their dysfunction in a variety of systemic diseases.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Thrombospondin-1
- CD47
- Red blood cells
- Calcium
- Cation channels
- Deformability
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Bissinger, Rosi
-
Petkova-Kirova, ...
-
Mykhailova, Olga
-
Oldenborg, Per-A ...
-
Novikova, Elena
-
Donkor, David A.
-
visa fler...
-
Dietz, Thomas
-
Bhuyan, Abdulla ...
-
Sheffield, Willi ...
-
Grau, Marijke
-
Artunc, Ferruh
-
Kaestner, Lars
-
Acker, Jason P.
-
Qadri, Syed M.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
- Artiklar i publikationen
-
Cell Communicati ...
- Av lärosätet
-
Umeå universitet