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Sökning: id:"swepub:oai:DiVA.org:umu-182002" > Deep mining of oxys...

Deep mining of oxysterols and cholestenoic acids in human plasma and cerebrospinal fluid : Quantification using isotope dilution mass spectrometry

Yutuc, Eylan (författare)
Swansea University Medical School, ILS1 Building, Singleton Park, Wales, Swansea, United Kingdom
Dickson, Alison L. (författare)
Swansea University Medical School, ILS1 Building, Singleton Park, Wales, Swansea, United Kingdom
Pacciarini, Manuela (författare)
Swansea University Medical School, ILS1 Building, Singleton Park, Wales, Swansea, United Kingdom
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Griffiths, Lauren (författare)
Swansea University Medical School, ILS1 Building, Singleton Park, Wales, Swansea, United Kingdom
Baker, Paul R.S. (författare)
Avanti Polar Lipids Inc., AL, Alabaster, United States
Connell, Lisa (författare)
Avanti Polar Lipids Inc., AL, Alabaster, United States
Öhman, Anders (författare)
Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
Forsgren, Lars (författare)
Umeå universitet,Neurovetenskaper
Trupp, Miles (författare)
Umeå universitet,Neurovetenskaper
Vilarinho, Sílvia (författare)
Departments of Internal Medicine, Section of Digestive Diseases, and of Pathology, Yale University School of Medicine, CT, New Haven, United States
Khalil, Youssef (författare)
Inborn Errors of Metabolism, Genetics and Genomic Medicine, UCL Great Ormond Street Institute of Child Health, 30 Guilford Street, London, United Kingdom
Clayton, Peter T. (författare)
Inborn Errors of Metabolism, Genetics and Genomic Medicine, UCL Great Ormond Street Institute of Child Health, 30 Guilford Street, London, United Kingdom
Sari, Sinan (författare)
Department of Pediatrics, Division of Gastroenterology, Gazi University Faculty of Medicine, Ankara, Turkey
Dalgic, Buket (författare)
Department of Pediatrics, Division of Gastroenterology, Gazi University Faculty of Medicine, Ankara, Turkey
Höflinger, Philip (författare)
Department of Neurology and Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany
Schöls, Ludger (författare)
Department of Neurology and Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany
Griffiths, William J. (författare)
Swansea University Medical School, ILS1 Building, Singleton Park, Wales, Swansea, United Kingdom
Wang, Yuqin (författare)
Swansea University Medical School, ILS1 Building, Singleton Park, Wales, Swansea, United Kingdom
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Swansea University Medical School, ILS1 Building, Singleton Park, Wales, Swansea, United Kingdom Avanti Polar Lipids Inc, AL, Alabaster, United States (creator_code:org_t)
Elsevier, 2021
2021
Engelska.
Ingår i: Analytica Chimica Acta. - : Elsevier. - 0003-2670 .- 1873-4324. ; 1154
Relaterad länk:
https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
https://doi.org/10.1...
https://urn.kb.se/re...
https://doi.org/10.1...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Both plasma and cerebrospinal fluid (CSF) are rich in cholesterol and its metabolites. Here we describe in detail a methodology for the identification and quantification of multiple sterols including oxysterols and sterol-acids found in these fluids. The method is translatable to any laboratory with access to liquid chromatography – tandem mass spectrometry. The method exploits isotope-dilution mass spectrometry for absolute quantification of target metabolites. The method is applicable for semi-quantification of other sterols for which isotope labelled surrogates are not available and approximate quantification of partially identified sterols. Values are reported for non-esterified sterols in the absence of saponification and total sterols following saponification. In this way absolute quantification data is reported for 17 sterols in the NIST SRM 1950 plasma along with semi-quantitative data for 8 additional sterols and approximate quantification for one further sterol. In a pooled (CSF) sample used for internal quality control, absolute quantification was performed on 10 sterols, semi-quantification on 9 sterols and approximate quantification on a further three partially identified sterols. The value of the method is illustrated by confirming the sterol phenotype of a patient suffering from ACOX2 deficiency, a rare disorder of bile acid biosynthesis, and in a plasma sample from a patient suffering from cerebrotendinous xanthomatosis, where cholesterol 27-hydroxylase is deficient.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

Bile acid
Cholestenoic acid
Cholesterol
Derivatisation
Hydroxycholesterol
Isotope-labelled standard
LC-MS

Publikations- och innehållstyp

ref (ämneskategori)
art (ämneskategori)

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