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Unveiling molecular...
Unveiling molecular interactions that stabilize bacterial adhesion pili
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- Dahlberg, Tobias, 1990- (author)
- Umeå universitet,Institutionen för fysik
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- Baker, Joseph (author)
- Department of Chemistry, The College of New Jersey, Ewing, New Jersey
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- Bullitt, Esther (author)
- Department of Physiology & Biophysics, Boston University School of Medicine, Boston, Massachusetts
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- Andersson, Magnus (author)
- Umeå universitet,Institutionen för fysik,Umeå Centre for Microbial Research (UCMR),The Biophysics and Biophotonics group
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(creator_code:org_t)
- Biophysical Society, 2022
- 2022
- English.
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In: Biophysical Journal. - : Biophysical Society. - 0006-3495 .- 1542-0086. ; 121:11, s. 2096-2106
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Adhesion pili assembled by the chaperone-usher pathway are superelastic helical filaments on the surface of bacteria, optimized for attachment to target cells. Here, we investigate the biophysical function and structural interactions that stabilize P pili from uropathogenic bacteria. Using optical tweezers, we measure P pilus subunit-subunit interaction dynamics and show that pilus compliance is contour-length dependent. Atomic details of subunit-subunit interactions of pili under tension are shown using steered molecular dynamics (sMD) simulations. sMD results also indicate that the N-terminal “staple” region of P pili, which provides interactions with pilins that are four and five subunits away, significantly stabilizes the helical filament structure. These data are consistent with previous structural data, and suggest that more layer-to-layer interactions could compensate for the lack of a staple in type 1 pili. This study informs our understanding of essential structural and dynamic features of adhesion pili, supporting the hypothesis that the function of pili is critically dependent on their structure and biophysical properties.
Subject headings
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- fysik
- Physics
Publication and Content Type
- ref (subject category)
- art (subject category)
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