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Selective isolation of hyaluronan by solid phase adsorption to silica

MacLeod, Rebecca (författare)
Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, 10010, NY, United States
Chan, Fok Vun (författare)
Echelon Biosciences Inc., 675 Arapeen Drive, Suite 302, Salt Lake City, 84108, UT, United States
Yuan, Han (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
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Ye, Xin (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
Sin, Yun Jin Ashley (författare)
Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, 10010, NY, United States
Vitelli, Teraesa M. (författare)
Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, 10010, NY, United States
Cucu, Tudor (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
Leung, Annie (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
Baljak, Irene (författare)
Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, 10010, NY, United States
Osinski, Samantha (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
Fu, Yuhong (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
Jung, Gyu Ik Daniel (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
Amar, Anant (författare)
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
DeAngelis, Paul L. (författare)
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 940 Stanton L. Young Blvd., Oklahoma, 73104, OK, United States
Hellman, Urban, 1966- (författare)
Umeå universitet,Avdelningen för medicin
Cowman, Mary K. (författare)
Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, 10010, NY, United States; Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States
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Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, 10010, NY, United States Echelon Biosciences Inc, 675 Arapeen Drive, Suite 302, Salt Lake City, 84108, UT, United States (creator_code:org_t)
Academic Press, 2022
2022
Engelska.
Ingår i: Analytical Biochemistry. - : Academic Press. - 0003-2697 .- 1096-0309. ; 652
Relaterad länk:
https://urn.kb.se/re...
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https://doi.org/10.1...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Nyckelord

Conditioned medium
Glycosaminoglycan
Hyaluronan
Isolation
Serum
Silica

Publikations- och innehållstyp

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