Regulation of PaRBOH1-mediated ROS production in Norway spruce by Ca2+ binding and phosphorylation

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Regulation of PaRBOH1-mediated ROS production in Norway spruce by Ca2+ binding and phosphorylation

Nickolov, Kaloian (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Department of Ecology and Genetics, University of Oulu, Oulu, Finland

Gauthier, Adrien (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; UniLaSalle, Agro-Ecology, Hydrogeochemistry, Environments & Resources, UP 2018.C101 of the Ministry in Charge of Agriculture (AGHYLE) Research Unit CS UP 2018.C101, Mont-Saint-Aignan, France

Hashimoto, Kenji (författare): Department of Applied Biological Science, Tokyo University of Science, Noda, Japan

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Laitinen, Teresa (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

Väisänen, Enni (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

Paasela, Tanja (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Natural Resources Institute Finland (Luke), Production Systems, Helsinki, Finland

Soliymani, Rabah (författare): Meilahti Clinical Proteomics Core Facility, Biochemistry & Dev. Biology, University of Helsinki, Biomedicum-Helsinki, Helsinki, Finland

Kurusu, Takamitsu (författare): Department of Applied Biological Science, Tokyo University of Science, Noda, Japan; Department of Mechanical and Electrical Engineering, Suwa University of Science, Chino, Japan

Himanen, Kristiina (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

Blokhina, Olga (författare): Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

Fagerstedt, Kurt V. (författare): Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

Jokipii-Lukkari, Soile (författare): Umeå universitet,Institutionen för fysiologisk botanik,Department of Ecology and Genetics, University of Oulu, Oulu, Finland

Tuominen, Hannele (författare): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology

Häggman, Hely (författare): Department of Ecology and Genetics, University of Oulu, Oulu, Finland

Wingsle, Gunnar (författare): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology

Teeri, Teemu H. (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

Kuchitsu, Kazuyuki (författare): Department of Applied Biological Science, Tokyo University of Science, Noda, Japan

Kärkönen, Anna (författare): Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Natural Resources Institute Finland (Luke), Production Systems, Helsinki, Finland

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Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; Department of Ecology and Genetics, University of Oulu, Oulu, Finland Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland; UniLaSalle, Agro-Ecology, Hydrogeochemistry, Environments & Resources, UP 2018C101 of the Ministry in Charge of Agriculture (AGHYLE) Research Unit CS UP 2018.C101, Mont-Saint-Aignan, France (creator_code:org_t)

2022-10-13
2022
Engelska.
Ingår i: Frontiers in Plant Science. - : Frontiers Media S.A.. - 1664-462X. ; 13

Relaterad länk:: https://doi.org/10.3...; visa fler...; https://umu.diva-por... (primary) (Raw object); https://pub.epsilon.... (primary) (Raw object) (free); https://urn.kb.se/re...; https://doi.org/10.3...; https://res.slu.se/i...; visa färre...

Tidskriftsartikel (refereegranskat)

Abstract Ämnesord

Stäng

Plant respiratory burst oxidase homologs (RBOHs) are plasma membrane-localized NADPH oxidases that generate superoxide anion radicals, which then dismutate to H2O2, into the apoplast using cytoplasmic NADPH as an electron donor. PaRBOH1 is the most highly expressed RBOH gene in developing xylem as well as in a lignin-forming cell culture of Norway spruce (Picea abies L. Karst.). Since no previous information about regulation of gymnosperm RBOHs exist, our aim was to resolve how PaRBOH1 is regulated with a focus on phosphorylation. The N-terminal part of PaRBOH1 was found to contain several putative phosphorylation sites and a four-times repeated motif with similarities to the Botrytis-induced kinase 1 target site in Arabidopsis AtRBOHD. Phosphorylation was indicated for six of the sites in in vitro kinase assays using 15 amino-acid-long peptides for each of the predicted phosphotarget site in the presence of protein extracts of developing xylem. Serine and threonine residues showing positive response in the peptide assays were individually mutated to alanine (kinase-inactive) or to aspartate (phosphomimic), and the wild type PaRBOH1 and the mutated constructs transfected to human kidney embryogenic (HEK293T) cells with a low endogenous level of extracellular ROS production. ROS-producing assays with HEK cells showed that Ca2+ and phosphorylation synergistically activate the enzyme and identified several serine and threonine residues that are likely to be phosphorylated including a novel phosphorylation site not characterized in other plant species. These were further investigated with a phosphoproteomic study. Results of Norway spruce, the first gymnosperm species studied in relation to RBOH regulation, show that regulation of RBOH activity is conserved among seed plants.

Ämnesord

NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)

Nyckelord

calcium ion
hydrogen peroxide
lignin formation
Norway spruce
phosphorylation
respiratory burst oxidase homolog (RBOH)

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Botanik

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Ekologi

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Av lärosätet: Umeå universitet; Sveriges Lantbruksuniversitet

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