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Metabolic reprogram...
Metabolic reprogramming and altered cell envelope characteristics in a pentose phosphate pathway mutant increases MRSA resistance to β-lactam antibiotics
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- Zeden, Merve S. (author)
- Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland
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- Gallagher, Laura A. (author)
- Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland
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- Bueno, Emilio (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Nolan, Aaron C. (author)
- Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland
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- Ahn, Jongsam (author)
- Department of Pathology and Microbiology, University of Nebraska Medical Center, NE, Omaha, United States
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- Shinde, Dhananjay (author)
- Department of Pathology and Microbiology, University of Nebraska Medical Center, NE, Omaha, United States
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- Razvi, Fareha (author)
- Department of Pathology and Microbiology, University of Nebraska Medical Center, NE, Omaha, United States
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- Sladek, Margaret (author)
- Department of Pathology and Microbiology, University of Nebraska Medical Center, NE, Omaha, United States
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- Burke, Órla (author)
- Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland
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- O'Neill, Eoghan (author)
- Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Dublin, Ireland
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- Fey, Paul D. (author)
- Department of Pathology and Microbiology, University of Nebraska Medical Center, NE, Omaha, United States
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- Cava, Felipe (author)
- Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Thomas, Vinai C. (author)
- Department of Pathology and Microbiology, University of Nebraska Medical Center, NE, Omaha, United States
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- O'Gara, James P. (author)
- Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland
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(creator_code:org_t)
- Public Library of Science (PLoS), 2023
- 2023
- English.
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In: PLoS Pathogens. - : Public Library of Science (PLoS). - 1553-7366 .- 1553-7374. ; 19:7
- Related links:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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Abstract
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- Central metabolic pathways control virulence and antibiotic resistance, and constitute potential targets for antibacterial drugs. In Staphylococcus aureus the role of the pentose phosphate pathway (PPP) remains largely unexplored. Mutation of the 6-phosphogluconolactonase gene pgl, which encodes the only non-essential enzyme in the oxidative phase of the PPP, significantly increased MRSA resistance to β-lactam antibiotics, particularly in chemically defined media with physiologically-relevant concentrations of glucose, and reduced oxacillin (OX)-induced lysis. Expression of the methicillin-resistance penicillin binding protein 2a and peptidoglycan architecture were unaffected. Carbon tracing and metabolomics revealed extensive metabolic reprogramming in the pgl mutant including increased flux to glycolysis, the TCA cycle, and several cell envelope precursors, which was consistent with increased β-lactam resistance. Morphologically, pgl mutant cells were smaller than wild-type with a thicker cell wall and ruffled surface when grown in OX. The pgl mutation reduced resistance to Congo Red, sulfamethoxazole and oxidative stress, and increased resistance to targocil, fosfomycin and vancomycin. Levels of lipoteichoic acids (LTAs) were significantly reduced in pgl, which may limit cell lysis, while the surface charge of pgl cells was significantly more positive. A vraG mutation in pgl reversed the increased OX resistance phenotype, and partially restored wild-type surface charge, but not LTA levels. Mutations in vraF or graRS from the VraFG/GraRS complex that regulates DltABCD-mediated d-alanylation of teichoic acids (which in turn controls β-lactam resistance and surface charge), also restored wild-type OX susceptibility. Collectively these data show that reduced levels of LTAs and OX-induced lysis combined with a VraFG/GraRS-dependent increase in cell surface positive charge are accompanied by significantly increased OX resistance in an MRSA pgl mutant.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
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- By the author/editor
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Zeden, Merve S.
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Gallagher, Laura ...
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Bueno, Emilio
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Nolan, Aaron C.
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Ahn, Jongsam
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Shinde, Dhananja ...
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show more...
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Razvi, Fareha
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Sladek, Margaret
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Burke, Órla
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O'Neill, Eoghan
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Fey, Paul D.
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Cava, Felipe
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Thomas, Vinai C.
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O'Gara, James P.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
- Articles in the publication
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PLoS Pathogens
- By the university
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Umeå University