Bactericidal effect of pulsed 1,064 nm Nd: YAG laser light on Staphylococcus epidermidis is of photothermal origin : an In Vitro Study

• Background and Objective: The aim was to investigate the bactericidal effect of the 1,064 nm Nd:YAG laser on Staphylococcus epidermidis.Material and Methods: S. epidermidis was inoculated on agar plates and then exposed to pulsed laser light in three different modes: with an uninterrupted train of pulses, or with two different repeated cycles of fractionated trains of pulses. The agar temperature was measured directly after uninterrupted radiation.Results: A bacterial growth inhibition area of 0.3 cm2 and maximum temperature of approximately 80 degrees C was observed after uninterrupted radiation at 2,000 J cm-2. The corresponding figures after an exposure of 5,000 J cm-2 were 0.9 cm2 and 100 degrees C, respectively. No bacterial inhibition was observed after exposure to repeated cycles of 20 seconds of radiation followed by 60 seconds of rest.Conclusion: The antimicrobial effect of the 1,064 nm Nd:YAG laser light is caused by a photothermal rather than a photochemical effect.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)

Nyckelord

agar

uninterrupted train of pulses

mode of radiation

temperature

EXCIMER-LASER

IN-VITRO

STERILIZATION

SURFACES

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