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Proximity labeling ...
Proximity labeling of host factor ANXA3 in HCV infection reveals a novel LARP1 function in viral entry
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- Bley, Hanna (författare)
- Institute of Virology, Philipps-University Marburg, Marburg, Germany
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- Krisp, Christoph (författare)
- Section Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
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- Schöbel, Anja (författare)
- Institute of Virology, Philipps-University Marburg, Marburg, Germany
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- Hehner, Julia (författare)
- Institute of Virology, Philipps-University Marburg, Marburg, Germany
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- Schneider, Laura (författare)
- Institute of Virology, Philipps-University Marburg, Marburg, Germany
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- Becker, Miriam (författare)
- Institute for Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hanover, Hanover, Germany
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- Stegmann, Cora (författare)
- Institute for Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hanover, Hanover, Germany
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- Heidenfels, Elisa (författare)
- Institute of Virology, Philipps-University Marburg, Marburg, Germany
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- Nguyen-Dinh, Van (författare)
- Institute of Virology, Philipps-University Marburg, Marburg, Germany
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- Schlüter, Hartmut (författare)
- Section Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
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- Gerold, Gisa, 1979- (författare)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Avdelningen för virologi,Institute for Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hanover, Hanover, Germany
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- Herker, Eva (författare)
- Institute of Virology, Philipps-University Marburg, Marburg, Germany
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(creator_code:org_t)
- Elsevier, 2024
- 2024
- Engelska.
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Ingår i: Journal of Biological Chemistry. - : Elsevier. - 0021-9258 .- 1083-351X. ; 300:5
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Hepatitis C virus (HCV) infection is tightly connected to the lipid metabolism with lipid droplets (LDs) serving as assembly sites for progeny virions. A previous LD proteome analysis identified annexin A3 (ANXA3) as an important HCV host factor that is enriched at LDs in infected cells and required for HCV morphogenesis. To further characterize ANXA3 function in HCV, we performed proximity labeling using ANXA3-BioID2 as bait in HCV-infected cells. Two of the top proteins identified proximal to ANXA3 during HCV infection were the La-related protein 1 (LARP1) and the ADP ribosylation factor-like protein 8B (ARL8B), both of which have been previously described to act in HCV particle production. In follow-up experiments, ARL8B functioned as a pro-viral HCV host factor without localizing to LDs and thus likely independent of ANXA3. In contrast, LARP1 interacts with HCV core protein in an RNA-dependent manner and is translocated to LDs by core protein. Knockdown of LARP1 decreased HCV spreading without altering HCV RNA replication or viral titers. Unexpectedly, entry of HCV particles and E1/E2-pseudotyped lentiviral particles was reduced by LARP1 depletion, whereas particle production was not altered. Using a recombinant vesicular stomatitis virus (VSV)ΔG entry assay, we showed that LARP1 depletion also decreased entry of VSV with VSV, MERS, and CHIKV glycoproteins. Therefore, our data expand the role of LARP1 as an HCV host factor that is most prominently involved in the early steps of infection, likely contributing to endocytosis of viral particles through the pleiotropic effect LARP1 has on the cellular translatome.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- Hepatitis C virus (HCV)
- host-pathogen interaction
- lipid droplets
- proteomics
- proximity labeling
- virus entry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Bley, Hanna
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Krisp, Christoph
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Schöbel, Anja
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Hehner, Julia
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Schneider, Laura
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Becker, Miriam
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visa fler...
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Stegmann, Cora
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Heidenfels, Elis ...
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Nguyen-Dinh, Van
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Schlüter, Hartmu ...
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Gerold, Gisa, 19 ...
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Herker, Eva
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Umeå universitet