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Optimization of pro...
Optimization of procedures for collecting and storing of CSF for studying the metabolome in ALS
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- Wuolikainen, Anna, 1980- (författare)
- Umeå universitet,Neurologi,Kemiska institutionen
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- Hedenström, Mattias (författare)
- Umeå universitet,Kemiska institutionen
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- Moritz, Thomas (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Marklund, Stefan (författare)
- Umeå universitet,Klinisk kemi
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- Antti, Henrik (författare)
- Umeå universitet,Kemiska institutionen
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- Andersen, Peter (författare)
- Umeå universitet,Neurologi
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(creator_code:org_t)
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- 2009-11-18
- 2009
- Engelska.
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Ingår i: Amyotrophic Lateral Sclerosis. - : Informa UK Limited. - 1748-2968 .- 1471-180X. ; 10:4, s. 229-236
- Relaterad länk:
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http://www.informawo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- There is a need for biomarkers for early diagnosis, development and evaluation of treatment efficacy in amyotrophic lateral sclerosis (ALS). We aimed to investigate if pre-analytical factors induce artefacts in metabolomic data of cerebrospinal fluid (CSF) from patients with ALS. CSF from 16 patients was studied using a statistical experimental design protocol with the following parameters: storage temperature (-80 degrees C/ - 20 degrees C), type of collection tube (polypropylene/polystyrene), and time delay from collecting to freezing (0, 10, 30, 90, 150 min). Gas chromatography-mass spectrometry was used to analyse CSF from 12 of the patients while CSF from one patient was analysed with nuclear magnetic resonance spectroscopy. The extent of CO(2) evaporization from CSF collected in tubes of different sizes at different temperatures and with/without lid were studied in three addtional patients. We found that alterations in storage temperature affect the metabolite composition of CSF more than any other studied pre-analytical parameter. CO(2) evaporization may induce artefacts in the metabolome by increasing the pH. In conclusion, minimization of evaluated artefacts can be obtained by collecting the CSF directly into tubes with tightly sealed lids in N(2)(l) and after freezing transfer of the tubes to -80 degrees C to minimize evaporation of CO(2).
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokemikalier (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biochemicals (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Nyckelord
- Cerebrospinal fluid
- metabolomics
- amyotrophic lateral sclerosis
- chemometrics
- CO2 evaporization
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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