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High-resolution thr...
High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas
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- Alanentalo, Tomas (författare)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Lorén, Christina E (författare)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Larefalk, Asa (författare)
- Umeå universitet,Medicinsk och klinisk genetik
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Sharpe, James (författare)
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- Holmberg, Dan (författare)
- Umeå universitet,Medicinsk och klinisk genetik
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- Ahlgren, Ulf (författare)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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(creator_code:org_t)
- Bellingham, WA : SPIE--the International Society for Optical Engineering, 2008
- 2008
- Engelska.
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Ingår i: Journal of Biomedical Optics. - Bellingham, WA : SPIE--the International Society for Optical Engineering. - 1083-3668 .- 1560-2281. ; 13:5, s. 054070-
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required.
Nyckelord
- biological organs
- biomedical optical imaging
- cellular biophysics
- diseases
- image resolution
- laser applications in medicine
- molecular biophysics
- optical tomography
- optical projection tomography
- confocal microscopy
- pancreas
- microbiopsy
- insulitis
- diabetes
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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