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[3H]GBR-12935 bindi...
[3H]GBR-12935 binding to human cerebral cortex is not to dopamine uptake sites.
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- Allard, Per (författare)
- Umeå universitet,Psykiatri
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Danielsson, M (författare)
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Papworth, K (författare)
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visa fler...
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Marcusson, J O (författare)
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visa färre...
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: Journal of Neurochemistry. - 0022-3042 .- 1471-4159. ; 62:1, s. 338-41
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
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- The binding of the dopamine uptake inhibitor [3H]GBR-12935 to 16 regions of the human brain was investigated in competition experiments with increasing concentrations of GBR-12909, mazindol, and dopamine. The methodology used included a relatively high tissue concentration (8 mg/ml) and addition of 5 mM KCl in the assay buffer. GBR-12909 inhibited 80-90% of the binding in most regions, whereas dopamine only inhibited the binding in the striatum. Mazindol inhibited only part of the cortical binding at concentrations of > 1 microM, whereas the inhibition in the caudate and the putamen also contained a high-affinity component representing the dopamine uptake site. It is concluded that the [3H]GBR-12935 binding sensitive to GBR-12909 cannot be regarded as specific binding to the dopamine uptake site because the displaceable binding most likely is not related to the dopamine uptake site.
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