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Modifying the cellu...
Modifying the cellular transport of DNA-based vaccines alters the immune response to hantavirus nucleocapsid protein
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Bucht, Göran (författare)
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Sjölander, Katarina Brus (författare)
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Eriksson, Solveig (författare)
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Lindgren, Lena (författare)
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Lundkvist, Åke (författare)
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- Elgh, Fredrik, 1957- (författare)
- Umeå universitet,Virologi,Fredrik Elgh
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Vaccine. - 0264-410X .- 1873-2518. ; 19:28-29, s. 3820-3829
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Puumala virus is a member of the hantavirus genus (family Bunyaviridae) and is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) in Europe. A genetic vaccination approach was conducted to investigate if the immune response could be modulated using different cellular secretion and/or localisation signals, and the immune responses were analysed in BALB/c mice and in a bank vole infectious model. Rodents vaccinated with DNA constructs encoding the antigen fused to an amino-terminal secretion signal raised significantly higher antibody levels when compared to using constructs lacking secretion signals. Furthermore, the ratios of the IgG subclasses (IgG2a/IgG1) were raised by the use of cellular localisation signals, indicating a more pronounced Th1-type of immune response. The majority of the mice, or bank voles, immunised with DNA encoding a secreted form of the antigen showed a positive lymphoproliferative response and were protected against challenge with Puumala virus (strain Kazan-wt).
Nyckelord
- Cellular transport; DNA vaccines; Hantavirus; Immune response; Puumala virus
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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