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Outer membrane vesi...
Outer membrane vesicle-mediated export of PrtV protease from Vibrio cholerae
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- Rompikuntal, Pramod Kumar (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Sun Nyunt Wai
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- Åhlund, Monika (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Lindmark, Barbro (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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visa fler...
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- Johnson, Tanya L (författare)
- Department of Microbiology and Immunology, University of Michigan Medical School.
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- Sandkvist, Maria (författare)
- Department of Microbiology and Immunology, University of Michigan Medical School.
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- Lundmark, Richard (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Uhlin, Bernt Eric (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Wai, Sun Nyunt (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Background: Gram-negative bacteria release large amounts of outer membrane vesicles (OMVs) during normal growth. OMVs from pathogenic bacteria are known to carry different biologically active toxins and enzymes into the surrounding environment. We hypothesized that OMVs may therefore be able to mediate the transport of bacterial products into host cells. We present here an analysis of the V. cholerae OMV-associated virulence factor PrtV. Methodology/Principal Findings: We observed that PrtV, a M6 family, zinc-binding metalloprotease, is secreted from V. cholerae wild type strain C6706 into the culture supernatant in association with OMVs. The association of PrtV with OMVs was determined by immunoblotting and electron microscopy using immunogold labeling. In addition, we observed that the PKD-domain(s) of PrtV has a role in the protein’s association with OMVs. We also demonstrated that OMV-associated PrtV was biologically active because HCT8 cells treated with OMVs from the wild type V. cholerae strain C6706 exhibited altered morphology, whereas cells treated with OMVs from the prtV isogenic mutant showed no morphological changes. Moreover, our data suggest that OMV-associated PrtV might be transported into target eukaryotic cells by a vesicle fusion mechanism in association with lipid raft microdomains in the plasma membrane.Conclusion/Significance: Our findings suggest that OMVs can deliver biologically active PrtV into target host cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- molekylärbiologi
- Molecular Biology
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)