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Differential expres...
Differential expression of interferon genes in a substrain of Namalwa cells.
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Lund, B (författare)
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von Gabain, A (författare)
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Edlund, T (författare)
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visa fler...
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- Ny, Tor (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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Lundgren, E (författare)
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visa färre...
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(creator_code:org_t)
- 1985
- 1985
- Engelska.
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Ingår i: Journal of interferon research. - 0197-8357. ; 5:2, s. 229-38
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- A substrain of Namalwa cells producing a high ratio of beta-interferon (IFN-beta) versus alpha-interferon (IFN-alpha) was investigated by constructing a cDNA library after induction with Sendai virus. The library was screened by two synthetic oligonucleotides, one specific for IFN-alpha and one complementary to both IFN-alpha and IFN-beta. Rescreening the library with two full-length cDNAs encoding IFN-alpha and IFN-beta, respectively, revealed that the frequency of the IFN-alpha and IFN-beta clones reflected the activities of IFN-alpha and IFN-beta obtained by functional assays. On the cDNA level, the dominating species was identical with the type of IFN-alpha A or IFN-alpha 2; however, one new type of cDNA also was found that was similar to the previously described IFN-alpha C. Only one type of cDNA was found encoding IFN-beta, although several IFN-beta proteins have been detected in the analyzed cell line.
Publikations- och innehållstyp
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- art (ämneskategori)
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