Sökning: id:"swepub:oai:DiVA.org:uu-103583" >
Neuropeptidomics :
Neuropeptidomics : expanding proteomics downwards.
-
- Svensson, Marcus (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
- Sköld, Karl (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
- Nilsson, Anna (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
visa fler...
-
- Fälth, Maria (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
Svenningsson, P. (författare)
-
- Andrén, Per E. (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
visa färre...
-
(creator_code:org_t)
- 2007
- 2007
- Engelska.
-
Ingår i: Biochemical Society Transactions. - 0300-5127 .- 1470-8752. ; 35:3, s. 588-593
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Biological function is mainly carried out by a dynamic population of proteins and peptides which may be used as markers for disease diagnosis, prognosis and as a guide for effective treatment. The study of proteins is called proteomics and it is generally performed by two-dimensional gel electrophoresis and mass spectrometric methods. However, gel-based proteomics is methodologically restricted from the low mass region, which includes important endogenous peptides. The study of endogenous peptides, peptidomics, is complicated by protein fragments produced post-mortem during conventional sample handling. Nanoflow liquid chromatography and MS, together with improved methods for sample preparation, have been used to semi-quantitatively monitor endogenous peptides in brain tissue. When rapidly heat-denatured brain tissue was analysed, these methods enabled simultaneous detection of hundreds of peptides and the identification of several endogenous peptides not previously described in the literature. In an application of the MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) model for Parkinson's disease, the expression of the small protein PEP-19 was compared with controls. The levels were found to be significantly decreased in the striatum of MPTP-treated animals.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Nyckelord
- Brain
- Degradation
- Matrix-assisted laser-desorption ionization (MALDI)
- Neuropeptide imaging
- Peptidomics
- Proteomics
- PHARMACY
- FARMACI
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas