Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken lines

• Background:Long-term selection (> 45 generations) for low or high   juvenile body weight from a common founder population of White Plymouth   Rock chickens has generated two extremely divergent lines, the LWS and   HWS lines. In addition to a > 9-fold difference between lines for the   selected trait, large behavioural and metabolic differences between the   two lines evolved during the course of the selection. We recently   compared gene expression in brain tissue from birds representing these   lines using a global cDNA array analysis and the results showed multiple but small expression differences in protein coding genes. The   main differentially expressed transcripts were endogenous retroviral   sequences identified as avian leucosis virus subgroup-E (ALVE).  Results: In this work we confirm the differential ALVE expression and   analysed expression and number of proviral integrations in the two   parental lines as well as in F-9 individuals from an advanced   intercross of the lines. Correlation analysis between expression,   proviral integrations and body weight showed that high ALVE levels in   the LWS line were inherited and that more ALVE integrations were   detected in LWS than HWS birds.Conclusion:We conclude that only a few of the integrations contribute   to the high expression levels seen in the LWS line and that high ALVE   expression was significantly correlated with lower body weights for the   females but not males. The conserved correlation between high   expression and low body weight in females after 9 generations of   intercrosses, indicated that ALVE loci conferring high expression directly affects growth or are very closely linked to loci regulating growth.

Ämnesord

LANTBRUKSVETENSKAPER  -- Veterinärmedicin (hsv//swe)

AGRICULTURAL SCIENCES  -- Veterinary Science (hsv//eng)

LANTBRUKSVETENSKAPER  -- Husdjursvetenskap (hsv//swe)

AGRICULTURAL SCIENCES  -- Animal and Dairy Sience (hsv//eng)

Nyckelord

MEDICINE

MEDICIN

Developmental Neurosciences

Medicinsk utvecklings- och neurobiologi

Publikations- och innehållstyp

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