Sökning: id:"swepub:oai:DiVA.org:uu-111550" >
The lipid peroxidat...
The lipid peroxidation metabolite 4-oxo-2-nonenal cross-links alpha-synuclein causing rapid formation of stable oligomers
-
- Näsström, Thomas (författare)
- Uppsala universitet,Geriatrik
-
- Wahlberg, Therese (författare)
- Uppsala universitet,Geriatrik
-
- Karlsson, Mikael (författare)
- Uppsala universitet,Mikrosystemteknik
-
visa fler...
-
- Nikolajeff, Fredrik (författare)
- Uppsala universitet,Mikrosystemteknik
-
- Lannfelt, Lars (författare)
- Uppsala universitet,Geriatrik
-
- Ingelsson, Martin (författare)
- Uppsala universitet,Geriatrik
-
- Bergström, Joakim (författare)
- Uppsala universitet,Geriatrik
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- Engelska.
-
Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 378:4, s. 872-876
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Recently, the aldehyde 4-oxo-2-nonenal (ONE) was identified as a product of lipid peroxidation and found to be an effective protein modifier. In this in vitro study we investigated structural implications of the interaction between ONE and alpha-synuclein, a protein which forms intraneuronal inclusions in neurodegenerative disorders such as Parkinson's disease and dementia with Lewy bodies. Our results demonstrate that ONE induced an almost complete conversion of monomeric alpha-synuclein into 40-80 nm wide and 6-8 nm high soluble beta-sheet-rich oligomers with a molecular weight of approximately 2000 kDa. Furthermore, the ONE-induced alpha-synuclein oligomers displayed a high stability and were not sensitive to treatment with sodium dodecyl sulfate, indicating that ONE stabilized the oligomers by cross-linking individual alpha-synuclein molecules. Despite prolonged incubation the oligomers did not continue to aggregate into a fibrillar state, thus suggesting that these alpha-synuclein species were not on a fibrillogenic pathway.
Nyckelord
- α-Synuclein
- 4-Oxo-2-nonenal
- Oligomers
- Parkinson’s disease
- Dementia with Lewy bodies
- Oxidative stress
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas