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Targeted resequenci...
Targeted resequencing of candidate genes using Selector Probes
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- Johansson, Henrik (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Isaksson, Magnus (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Sörqvist, Elin Falk (author)
- Uppsala universitet,Molekylära verktyg,Forskargrupp Mats Nilsson
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- Roos, F. (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Stenberg, Johan (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Sjöblom, Tobias (author)
- Uppsala universitet,Genomik,Forskargrupp Sjöblom
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- Botling, Johan (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Forskargrupp Botling
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- Micke, Patrick (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Forskargrupp Botling
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- Edlund, Karolina (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Forskargrupp Botling
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Fredriksson, S. (author)
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- Göransson Kultima, Hanna (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Cancer Pharmacology and Computational medicine
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- Ericsson, Olle (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Nilsson, Mats (author)
- Uppsala universitet,Molekylära verktyg,Forskargrupp Mats Nilsson
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(creator_code:org_t)
- 2010-11-08
- 2011
- English.
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In: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 39:2, s. e8-
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Targeted genome enrichment is a powerful tool for making use of the massive throughput of novel DNA-sequencing instruments. We herein present a simple and scalable protocol for multiplex amplification of target regions based on the Selector technique. The updated version exhibits improved coverage and compatibility with next-generation-sequencing (NGS) library-construction procedures for shotgun sequencing with NGS platforms. To demonstrate the performance of the technique, all 501 exons from 28 genes frequently involved in cancer were enriched for and sequenced in specimens derived from cell lines and tumor biopsies. DNA from both fresh frozen and formalin-fixed paraffin-embedded biopsies were analyzed and 94 specificity and 98 coverage of the targeted region was achieved. Reproducibility between replicates was high (R 2=0, 98) and readily enabled detection of copy-number variations. The procedure can be carried out in <24 h and does not require any dedicated instrumentation.
Keyword
- Resequencing
- Targeted
- Next generation sequencing
- Selectors
- Cancer
- Tumor
- FFPE
- MEDICINE
- MEDICIN
- Medical Genetics
- Medicinsk genetik
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Johansson, Henri ...
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Isaksson, Magnus
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Sörqvist, Elin F ...
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Roos, F.
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Stenberg, Johan
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Sjöblom, Tobias
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show more...
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Botling, Johan
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Micke, Patrick
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Edlund, Karolina
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Fredriksson, S.
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Göransson Kultim ...
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Ericsson, Olle
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Nilsson, Mats
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show less...
- Articles in the publication
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Nucleic Acids Re ...
- By the university
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Uppsala University