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Twelve-gel slide fo...
Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation
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Shaposhnikov, Sergey (author)
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Azqueta, Amaya (author)
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- Henriksson, Sara (author)
- Uppsala universitet,Institutionen för genetik och patologi
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Meier, Silja (author)
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Gaivão, Isabel (author)
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Huskisson, Neville H. (author)
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Smart, Andrew (author)
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Brunborg, Gunnar (author)
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- Nilsson, Mats (author)
- Uppsala universitet,Institutionen för genetik och patologi
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Collins, Andrew R. (author)
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(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- English.
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In: Toxicology Letters. - : Elsevier BV. - 0378-4274 .- 1879-3169. ; 195:1, s. 31-34
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The comet assay is widely used to measure DNA damage and repair in basic research, genotoxicity testing and human biomonitoring. The conventional format has 1 or 2 gels on a microscope slide, 1 sample per slide. To increase throughput, we have designed and tested a system with 12 smaller gels on one slide, allowing incubation of individual gels with different reagents or enzymes. Thus several times more samples can be analysed with one electrophoresis run, and fewer cells and smaller volumes of test solutions are required. Applications of the modified method include treatment with genotoxic agents at different concentrations; simultaneous analysis of different lesions using a range of enzymes; analysis of cell extracts for DNA repair activity; and fluorescent in situ hybridisation (FISH) to comet DNA with specific labelled probes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Keyword
- Molecular medicine (genetics and pathology)
- Molekylär medicin (genetik och patologi)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Shaposhnikov, Se ...
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Azqueta, Amaya
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Henriksson, Sara
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Meier, Silja
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Gaivão, Isabel
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Huskisson, Nevil ...
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show more...
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Smart, Andrew
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Brunborg, Gunnar
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Nilsson, Mats
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Collins, Andrew ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
- Articles in the publication
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Toxicology Lette ...
- By the university
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Uppsala University