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Ryanodine receptor-...
Ryanodine receptor-operated activation of TRP-like channels can trigger critical Ca2+ signaling events in pancreatic beta-cells
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Gustafsson, Amanda Jabin (författare)
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Ingelman-Sundberg, Hanna (författare)
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- Dzabic, Mensur (författare)
- Karolinska Institutet
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Awasum, Justina (författare)
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Nguyen, Khanh Hoa (författare)
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- Östenson, Claes-Göran (författare)
- Karolinska Institutet
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Pierro, Cristina (författare)
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Tedeschi, Patrizia (författare)
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Woolcott, Orison (författare)
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- Chiounan, Shiue (författare)
- Karolinska Institutet
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Lund, Per-Eric (författare)
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Larsson, Olof (författare)
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- Islam, M. Shahidul (författare)
- Karolinska Institutet,Uppsala universitet,Medicin
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(creator_code:org_t)
- 2004-11-30
- 2005
- Engelska.
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Ingår i: The FASEB Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 19:2, s. 301-303
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- There is little information available concerning the link between the ryanodine (RY) receptors and the downstream Ca(2+) signaling events in beta-cells. In fura-2 loaded INS-1E cells, activation of RY receptors by 9-methyl 5,7-dibromoeudistomin D (MBED) caused a rapid rise of [Ca(2+)]i followed by a plateau and repetitive [Ca(2+)]i spikes on the plateau. The [Ca(2+)]i plateau was abolished by omission of extracellular Ca(2+) and by SKF 96365. In the presence of SKF 96365, MBED produced a transient increase of [Ca(2+)]i, which was abolished by thapsigargin. Activation of RY receptors caused Ca(2+) entry even when the ER Ca(2+) pool was depleted by thapsigargin. The [Ca(2+)]i plateau was not inhibited by nimodipine or ruthenium red, but was inhibited by membrane depolarization, La(3+), Gd(3+), niflumic acid, and 2-aminoethoxydiphenyl borate, agents that inhibit the transient receptor potential channels. The [Ca(2+)]i spikes were inhibited by nimodipine and ryanodine, indicating that they were due to Ca(2+) influx through the voltage-gated Ca(2+) channels and Ca(2+)-induced Ca(2+) release (CICR). Activation of RY receptors depolarized membrane potential as measured by patch clamp. Thus, activation of RY receptors leads to coherent changes in Ca(2+) signaling, which includes activation of TRP-like channels, membrane depolarization, activation of the voltage-gated Ca(2+) channels and CICR.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Gustafsson, Aman ...
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Ingelman-Sundber ...
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Dzabic, Mensur
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Awasum, Justina
-
Nguyen, Khanh Ho ...
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Östenson, Claes- ...
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visa fler...
-
Pierro, Cristina
-
Tedeschi, Patriz ...
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Woolcott, Orison
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Chiounan, Shiue
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Lund, Per-Eric
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Larsson, Olof
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Islam, M. Shahid ...
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visa färre...
- Artiklar i publikationen
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The FASEB Journa ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet